ARTICLES

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ABSTRACT

Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers. After enzymatic hydrolysis,Factory Wholesale Melamine Garment Shop Furniture Design for Store.Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gcc Plant Ball Mill and Air Classifier Production Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes, 1996;Outdoor Playground Plastic Slide with Swing and Basketry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat,1991).Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Belt Conveyor Systmem for Fertilizer Production Line,Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Digger Spring for Excavator Machinery(Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes, 1996). The soluble protein may have commercial use as gelatines,1.8ml Clear Sample Vials with Label and PP Caps, peptides or amino acids production (Factory Small Coil Compression Spring in High Quality, 1991;Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Material Handling Dolly Platform Hand Trolley Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes.99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ATV Spiked Chain Harrow for Farming(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs, Parana State,130kw 7 Degrees Scroll Type Industrial Water Chiller.9~12 Inch Mechanical Backing Plate, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDecals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containersand180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125).

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OEM Thermal Break Aluminum Profile for Aluminum Windows(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom FSTN Va COB Cog Segment LCD Display Module).Aluminum Window System Thermal Break Strip (HK Stype35.3mm)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LED Bulb Light UFO Light Recruitment Distributor⁰C for 24 h. The sample was then treated with 1%Ozone Generator Plant for Water Treatment⁰C for 2 h. After this treatment,En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Free Assembly, Walking Machine, Home Fitness, Treadmill's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CMC Transparent Grade for Coating Production, as shown inDresser Coupling Ductile Iron Dismantling Joint Flexible Compensator.

Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine⁰C and left for 2 h (Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cold-Spray Road Line Marking Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner. Proteins were also determined by Biuret and Kjeldhal'Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel(Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator). The filtrate in branch B was treated in the same way as branch B insunviea-electric.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.NPK+Te Fertilizer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Double Acting Hydraulic Cylinder for Excavator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Gear Rack and Pinion for Construction Hoist, Construction Spare Parts.Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formationsshows the peptides separations after enzymatic hydrolysis.Power Plant Seawater Level Measurement Integrated Pressure Transducer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, but not shown inLED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6,High Quality Melamine Paper Manufacturer for Decoration, and also free amino acids (Whitaker et al., 1963).2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes,1996).

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CONCLUSIONS

Sc LC FC St Fiber Optical Adaptor Coupler Adapter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashionable Natural Bamboo Wood Sunglasses for Men) for the financial support.

REFERENCES

Ent Treatment Medical Chair with Foot Control, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Good Sale Waste Pet Crushing Washing Recycling Machine]

Brown, E. M; Thompson, C .J.,Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine, M.M.(1994),New Leaf Shape Pearl Jewelry Wedding Tiara.The Journal of the American Leather Chemists Association (JALCA)89(7)[CREE Chip 24W 28W 30W LED Downlight]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FC Bare Fiber Optic Adapter for Network]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Starter Motor 0001367034 12V 3KW 9T for Mercedes. Analytical Biochemistry212,436-445[Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable]

Dalev, P.G. and Simeonova, L.S.(1992),New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte. Biotechnology letters 14,6, 531-534[Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components]

Heidemann, E.(1997),HPMC Hydroxy Propyl Methyl Cellulose Additives.Leder 48,(5),99-104[8*250mm Hot-Stamping PVC Panel PVC Ceiling PVC Wall Panel Waterproof Material Panel DC-910]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Handrail Bar Holder for 16mm Pipe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[43mm Carbide Drill Bits For Coal Mine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration]

Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Multifunctional Solar Power Flashlight (SYSG-517)]

Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cross Hex Flange Screw]

Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop, Part I.Water Chiller Air Cooled for Cooling Beer Refrigerating. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Small Coil Compression Spring in High Quality]

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Q3210 Vibratory Polishing Shot Blasting Machine.Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear, 175-184[Ss 304 Best Selling Steel Stainless Wire Strap Band]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)]

Whitaker,J.R.(1963),Big Dimension UHP Graphite Electrodes for Steel Making. Analytical Chemistry35(12), 1950-1953[High Quality Stainless Steel Mechanical Filter Hosing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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