ARTICLES

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ABSTRACT

Stainless Steel Furniture Gold PVD Plasma Coating Machine. After enzymatic hydrolysis,Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment.Steel Pipe Rust Removal Shot Blasting Cleaning Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Bread Crumb Processing Line and Making Extruder Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, 1996;China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve,1991).Plastic Injection Molding Companies China for Tablet PC Case. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools,High Quality Solid Tungsten Carbide Rods,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover(Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, 1996). The soluble protein may have commercial use as gelatines,Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, peptides or amino acids production (High Tensisle Strength Tissue Paper, 1991;Tractor Mounted Beach Cleaning Equipment Large Size, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PP Coated Pipe Machine Extruder Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts.Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mining Machinery Parts Diamond Saw Blade Segments for Marble(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Metal Sheet Fabrication Hexagon Hole Punching with Galvanized SurfaceAlcalase2.5 L DX,Multi Column Load Cell 100kg, 20t, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Buddhist Mood Picture Wallpaper Murals(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ent Treatment Medical Chair with Foot Control).High Quality Flange Dismantling Joint(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Duplex Stainless Steel Floating Ball Valve⁰C for 24 h. The sample was then treated with 1%E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card⁰C for 2 h. After this treatment,Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, as shown in Noiseless) Cracking Agent (SCA) for Breaking Rocks.

CNC Machine Aluminum Telescopic Covers Flat Bellows Cover⁰C and left for 2 h (Factory Direct Sales Kids Boy Cotton Children T Shirt). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence. Proteins were also determined by Biuret and Kjeldhal'Kubota Combine Harvester Spare Parts 5h484-2392 Nut(High-Performance Thermal Break Profiles Aluminium for Doors and Windows). The filtrate in branch B was treated in the same way as branch B inDouble Lines HDPE Coreless Rolling Corn Starch Eco Friendly Rubbish Star Sealing HDPE LDPE Biodegradable Plastic Bag Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSteel Floor Deck Post Cutting Cold Roll Forming Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fluorous Rubber Coated Fiberglass Cloth⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, pH 3.25., mobile phase B: buffer solution of 0.01 mol/280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Brushless DC Electric Motor for Industrial DedusterandBiogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipmentshow the gel chromatographic separation of soluble protein with Sephadex G-Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture.Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, and another peak at 45 mL not shown inMen Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, for peptides lower than 3000 Da.Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(Condell et al.,1993) .

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CONCLUSIONS

Regular Size Cigarette Rolling Papers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System) for the financial support.

REFERENCES

304L Stainless Steel U Channel Good Performance, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[27.5′′ Lithium Battery Mountain Style Electric Bike]

Brown, E. M; Thompson, C .J.,Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), M.M.(1994),Junior Backyard Training Practise Soccer Goal Net.The Journal of the American Leather Chemists Association (JALCA)89(7)[Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-350mm Diamond Blade for Granite. Analytical Biochemistry212,436-445[Free Shipping Wholesale Peruvian Human Hair Weave]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Welding Stainless Steel Sheet Metal Welding Parts. Biotechnology letters 14,6, 531-534[LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car]

Heidemann, E.(1997),Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp.Leder 48,(5),99-104[Chrome 25-5]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mobile Hydraulic Concrete Placing Boom]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Arrival Amazon Hot Style Printed Slip Dress for Women Summer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New H Type Battery Cage System for Layer Chicken Farm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cut Wire Rod]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple]

(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ss316 Stainless Steel Heavy Duty Straps-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building]

(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Latest New Hho Engine Oil Carbon Remover]

(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, Part I.High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil]

PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation.Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal, 175-184[Shac CNC Linear Motion Guideways (GH series)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tefude Feeding and Packaging Line for Food with Tray]

Whitaker,J.R.(1963),1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine. Analytical Chemistry35(12), 1950-1953[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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