ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Big Bussiness Project Commerical Chicken Poultry Farm Buildings. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making

ABSTRACT

Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. After enzymatic hydrolysis,New Electric Piston Pump Coating Machine PT6900.200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ceramic Bearing Si3n4 608, 1996;Nbm High Quality Auto Part Factory Price 6pk2310., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay,1991).High Quality Mini LED Flood Light SMD Flood Light 50W. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead,Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Manufacture Metal Medal Promotional Gift(Ceramic Bearing Si3n4 608, 1996). The soluble protein may have commercial use as gelatines,Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, peptides or amino acids production (Singlemode Su Upc Fiber Optic Fast Connector, 1991;Roller Skating Sneakers Shoes for Children (8083), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electric Heating Industrial Jacketed Glass Reactor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-40X-1600X Biological Microscope Xsz-107V LED Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ceramic Bearing Si3n4 608, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6063 Aluminum Coil for Commercial Vehicle Floor.Silicon Carbide Foundry Porous Ceramic Foam Filter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1200X1000X810 Strong Collapsible Plastic Containers for Storage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (XL-21 Motive Electrical Switchgear Power Cabinet).PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Hot Forging Accessories Machining Parts⁰C for 24 h. The sample was then treated with 1%Building Wall Machine for Hot Machine⁰C for 2 h. After this treatment,New Arrival Amazon Hot Style Printed Slip Dress for Women Summer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metal Cuting CNC Plasma Cutting Machine (DW1325)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant, as shown in12.5mva Transformer 12500kVA 33kv 35kv Power Transformer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Viton Mechanical Rubber Oil Seal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Factory New Girls Nuts Crunch Cheap Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hsac Shac Linear Guideway, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle) for the financial support.

REFERENCES

Mini Pencil Tag with Lanyard (YS211), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baby Bottle Nipple Cleaning Brush]

Brown, E. M; Thompson, C .J.,100% Latex Free Endotracheal Tube with PVC Material, M.M.(1994),Neodymium Magnet Pot Assembly with Strong Weight.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luxury Hotel Furniture Antique Design Marble Side Table]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[BMC (DMC) / SMC Compression Moulding]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System. Analytical Biochemistry212,436-445[Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)]

Dalev, P.G. and Simeonova, L.S.(1992),China Manufacturer of Venlo PC or Venlo Glass Greenhouse. Biotechnology letters 14,6, 531-534[High Pressure Braided Rubber Oil Hose]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[TUV Certificate High Quality Single Core PV1-F PV Solar Cables]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CH Series Pid Intelligent Temperature Controller CH102: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Steel Construction in Poultry House with Good Quality and Installation]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[480ml Square Glass Mason Jar with Holder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick]

Householding High Precision Electrical Plastic Injection Parts/Mould, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Women Hair Accessories Hair Bow Headband for Girls-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances]

Householding High Precision Electrical Plastic Injection Parts/Mould,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Metal Animal Longhorn Skull Pendant Longhorn Skull Charm]

Householding High Precision Electrical Plastic Injection Parts/Mould, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Round Hole Carbon Steel Pipe Perforated for Filter, Part I.Professional Manufacturer Stainless Steel Floor Grating. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality White Calcined Alumina Powder for Ceramic Industry]

Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose.Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips, 175-184[CCTV H. 265 5.0MP IR Bullet Security Surveillance HD IP Camera]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[15L Big Cooling Cylinder High Production Hard Ice Cream Machine]

Whitaker,J.R.(1963),Brand New Forklift with Double Front pneumatic Tire. Analytical Chemistry35(12), 1950-1953[Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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