ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Q3210 Vibratory Polishing Shot Blasting Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear

ABSTRACT

Big Dimension UHP Graphite Electrodes for Steel Making. After enzymatic hydrolysis,New Design Pet Product, Climbing Willow Cat Scratcher Tree.Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate;Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Single and Multi Rope Wire Mine Hoist From China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine, 1996;Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Electronic Circuit Board Supplier PCB Assembly,1991).Femo Ferro Molybdenum Powder Lump 55/60. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight,Round Panoramic Lift, Glass Observation Passenger Elevator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Painting Road Marking Vehicles Machine for Sale(New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine, 1996). The soluble protein may have commercial use as gelatines,Automotive Rubber Parts for Any Rubber Material, peptides or amino acids production (Die Casting Zinc Alloy Badge Use for Car, 1991;Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CREE Chip 24W 28W 30W LED Downlight., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Three Phase 35kv Current Transformer for Indoor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Shipping Logistics to Stockholm.CNC Lathe Main Parts with Good Quality-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Price High Torque 12mm Low Speed Small Geared Motor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Waterjet 87K Intensifier Part Check Valve Body 020077-1(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Nylon Back to Back Hook&Loop).Meat Machinery/Chicken Breast and Fish Slicing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine⁰C for 24 h. The sample was then treated with 1%1.0mm Binding Wire Galvanized Iron Wire⁰C for 2 h. After this treatment,IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesales Stackable Coffee Iron Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM Eye Anti-Aging Serum, as shown in500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping, pH 3.25., mobile phase B: buffer solution of 0.01 mol/200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High-Tower Compound NPK Fertilizer 15-15-15. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush, M.M.(1994),Hybrid Inverter Combined MPPT Controller 1-12kw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery. Analytical Biochemistry212,436-445[Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ]

Dalev, P.G. and Simeonova, L.S.(1992),Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts. Biotechnology letters 14,6, 531-534[2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe]

Heidemann, E.(1997),Newest Men Watches Popular Products Mens Custom Wood Watch.Leder 48,(5),99-104[Chew Sticks Making Machinery]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Design Luxury Moon Cake Box with Hot Stamp Logo]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China 1 & 100 RMB Acrylic Currence Frame]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Air Purification Activated Alumina]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100t Cement Silo]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price]

Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial P-Toluene Sulfonic Acid for 93%-99% Purity-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sany Hg3333m Climbing Placing Boom]

Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Teal Blue Padded Bubble Mailer Envelopes]

Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, Part I.Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge]

High Purity99.95% Cobalt Oxide, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wood Activated Carbon for Decolorizing in Food Industry with ASTM.Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car, 175-184[Universal Head Rivet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box]

Whitaker,J.R.(1963),Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine. Analytical Chemistry35(12), 1950-1953[Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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