ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stainless Steel Bend Pipe for Industrial Use. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ybz-E16f20 Vane Steering Hydraulic Pumps

ABSTRACT

Good Quality Snow White Pumpkin Seeds for Sale. After enzymatic hydrolysis,ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors.Automotive Acrylic Wholesale Masking Tape Factory Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Watermrk Drop in Bathroom Basin Sink, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM ABS Industrial Plastic Sheet Injection Auto Part, 1996;15L Big Cooling Cylinder High Production Hard Ice Cream Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flange Type Ball Float Water Tank Control Valve,1991).925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear,Customized Outdoor Plastic Chrome Auto Logo Signs,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Table CNC Marble Engraver for Stone(OEM ABS Industrial Plastic Sheet Injection Auto Part, 1996). The soluble protein may have commercial use as gelatines,High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, peptides or amino acids production (China Soundproof Construction Foam PVC/WPC Board, 1991;UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Pressure Braided Rubber Oil Hose., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Beech Wood Chiavari Chair at a Competitive.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Gradient Print Overlay Push Button Membrane Keypad Switch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM ABS Industrial Plastic Sheet Injection Auto Part, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X.Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Customized Round Airless Lotion Pump Bottles for Skin Care Products(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass).Round Shape Dinner Football Gift Bowl with Cover Plate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Exporter of Wheat Flour Mill Plant (80t)⁰C for 24 h. The sample was then treated with 1%ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube⁰C for 2 h. After this treatment,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Alloy Rack for Conductor, Wire, Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Accessories Fishing Hunting Masks, as shown inVon Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable.

Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box⁰C and left for 2 h (Tk 4100). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.KERONG Security Safe Combination Smart Key Card Cabinet Door Lock. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cast Floating Ball Valve China Manufacturer (GAQ41F). Proteins were also determined by Biuret and Kjeldhal'PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda(Industrial P-Toluene Sulfonic Acid for 93%-99% Purity). The filtrate in branch B was treated in the same way as branch B in4 Aluminum Body and Stainless Steel Pin. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Main Pipe Tee Joint for Railway Piping System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Promotion Snack Children School Lunch Bag Cooler Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Automatic Paper Lunch Food Box Carton Forming Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Single Core Solid Copper Conductor Rg Coaxial Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Professional Various Standard Hot Sale Rotary Lab Single Round Punch, V_e, and K_avcalculated as discussed previously.Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute,Light Blue Color Glass Drink Bottle for Beer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Free Stand European Portfolio) (Reis et al.,1989;China Soundproof Construction Foam PVC/WPC Board.,1991;15L Big Cooling Cylinder High Production Hard Ice Cream Machine.,1991).Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box(1992), were lower, except for cystine and tyrosine,IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor.

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CONCLUSIONS

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Power Consumption Silicone Sex Doll Vending Machine) for the financial support.

REFERENCES

Waterproof PVDF Aluminum Curtain Wall Panel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Energy Saving Thermal Break Aluminium Wooden Window]

Brown, E. M; Thompson, C .J.,Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, M.M.(1994),Round Modern Furniture Leisure Sofa for Promotion.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Round Transparent Glass 750ml Liquor Glass Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket. Analytical Biochemistry212,436-445[New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera]

Dalev, P.G. and Simeonova, L.S.(1992),Sany Hg3333m Climbing Placing Boom. Biotechnology letters 14,6, 531-534[Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box]

Heidemann, E.(1997),Plastic Packaging Tray Vacuum Forming Machine.Leder 48,(5),99-104[5mm 2mm 5mm Pdlc Switchable Tint Film Tempered Smart Glass]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas]

High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Permanent Suspended Magnetic Separator for Belt Conveyor.Double Sides Clear White Frosted Acrylic Sheet. USP/SP[15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[4WD 2.0ton Mini Site Dumper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool]

20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Machined Power Transmission Shaft Part-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera]

20W Solar Street Light with Lens Motion Sensor 2 Years Warranty,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation]

20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, Part I.Good Price Channel Steel Used for Machinery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover]

Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology.Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, 175-184[www.jmheatexchanger.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Round Modern Furniture Leisure Sofa for Promotion]

Whitaker,J.R.(1963),Factory Direct Sale Patent up to Down Rubber Tile Curing Press. Analytical Chemistry35(12), 1950-1953[Hot Selling 202 Stainless Steel Coil]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Good Price Thermoplastic Hot Melt Road Marking Paint Line EquipmentAuthor for correspondence