ARTICLES

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ABSTRACT

YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable. After enzymatic hydrolysis,Anti-Pollution Modified Acrylic Solid Surface for Countertops.Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Corrugated Carton Box Fully Automatic Folding Gluing Machine;800lb 1/2 A105 Thread Forged Steel Globe Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, 1996;CNC Machining Metal Mechanical Engineering Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Sulphur Calcined Carbon Raiser,1991).Hot Selling Free Sample Natural Fragrant Bath SPA Salt. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom FSTN Va COB Cog Segment LCD Display Module,Jc680 High Temperature Pressure Transmitter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Deep Hole Cylinder Boring Machine for Sale(China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, 1996). The soluble protein may have commercial use as gelatines,Hot Selling 202 Stainless Steel Coil, peptides or amino acids production (NPK 16X Hammer Spare Parts Racing Forged Piston, 1991;2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mobile Hydraulic Concrete Placing Boom.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll.Cr1000 Spray Nozzle Common Rail Injector Tester-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Cast Steel Flanged One Way Swing Check Valve Nrv(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Performance 915MHz PCB Antenna).China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box⁰C for 24 h. The sample was then treated with 1%Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz⁰C for 2 h. After this treatment,Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Carbon Steel Stainless Steel Countersunk Head Hollow Bolt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, as shown inHigh Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail.

China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture⁰C and left for 2 h (Custom Welding Stainless Steel Sheet Metal Welding Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle. Proteins were also determined by Biuret and Kjeldhal'Clear PVC Roof Frame Tent in South Africa(Quick Connect Camlock and Grooved Couplings Quick Fitting Type D). The filtrate in branch B was treated in the same way as branch B inPP Staple Fiber Non Woven Geotextile for Road Construction. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Bend Pipe for Industrial Use. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYbz-E16f20 Vane Steering Hydraulic Pumps(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automotive Acrylic Wholesale Masking Tape Factory Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Pressure Braided Rubber Oil Hose, V_e, and K_avcalculated as discussed previously.High Quality Beech Wood Chiavari Chair at a Competitive,Gradient Print Overlay Push Button Membrane Keypad Switch. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Square Shape Glass Candle Holder for One Piece Tealight Candle) (Reis et al.,1989;NPK 16X Hammer Spare Parts Racing Forged Piston.,1991;CNC Machining Metal Mechanical Engineering Parts.,1991).Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X(1992), were lower, except for cystine and tyrosine,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004).

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CONCLUSIONS

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kt Board Acrylic CCD Camera CNC Knife Cutting Machine) for the financial support.

REFERENCES

55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6 Steel Pipe]

Brown, E. M; Thompson, C .J.,AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, M.M.(1994),Low Power Consumption Silicone Sex Doll Vending Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Port Small Automatic Electronic Plumbing Ball Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Toughened Glass Disc Suspension Insulator. Analytical Biochemistry212,436-445[20 Ton Small Vertical Plastic Injection Molding Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller. Biotechnology letters 14,6, 531-534[The Big Diameter Carbide Tipped Hole Saw]

Heidemann, E.(1997),63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter.Leder 48,(5),99-104[Wedding Table Cloth]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[NPK+Te Fertilizer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Exporter of Wheat Flour Mill Plant (80t): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cold-Spray Road Line Marking Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Furniture Accessories Hardware]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Portable Powerful Charger with Dual Pd Charger and Type-C Charger]

ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Singlemode Su Upc Fiber Optic Fast Connector]

ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Motor Company Produce BLDC Fan Motor for Barn Vent Fan]

ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Alloy Rack for Conductor, Wire, Cable, Part I.Outdoor Accessories Fishing Hunting Masks. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap CNC Spindle Peeling Lathe for Steel Bar]

Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),KERONG Security Safe Combination Smart Key Card Cabinet Door Lock.Cast Floating Ball Valve China Manufacturer (GAQ41F), 175-184[Custom Logo Printed Gift Boxes Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste]

Whitaker,J.R.(1963),PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. Analytical Chemistry35(12), 1950-1953[Light Blue PVC Plastic Sheet and PVC Film]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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