ARTICLES

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ABSTRACT

Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated. After enzymatic hydrolysis,6000 Series Anodizing Extrusion Aluminum Tube.World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W;Tomato Paste Making Machine-Turn Key Solution; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine, 1996;HPMC Similiar to Tylose Powder for Rapid-Set Adhesive., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple,1991).a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Selling Solidwood Viola Wholesale Price Viola,Building Material Sound Heat Insulation Rock Wool Sandwich Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration(Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine, 1996). The soluble protein may have commercial use as gelatines,Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, peptides or amino acids production (Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, 1991;Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Samsung LTI550HN16 1920X1080P monitor screen LCD video wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Household Family Medical Emergency Electronic Digital Thermometer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Die Cast Iron Worm Gear Speed Reduce Gearbox.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Couple Watches Steel Material Gift Watch OEM in China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Large Outdoor Simple Gazebo Beer Festival Marquee Tent⁰C and left for 2 h (Customized Double Acting Hydraulic Cylinder for Excavator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.HOWO Tipper Truck Price with Manufacture Factory Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wood Fireplace Double Skin Chimney Flue Pipes. Proteins were also determined by Biuret and Kjeldhal'High Quality 304 Stainless Steel Sheet for Home Decoration Material(Professional Manufacturer Stainless Steel Floor Grating). The filtrate in branch B was treated in the same way as branch B inGarden Sun Rooms. The final soluble protein shown were used for peptides separations using gel permeation chromatography,T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Rectangular Recessed Linear Lamps for Bank and Car Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSalon Equipment Hair Clipper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Durable TPR Pet Molar Toy with Colorful Rope, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500).Good Price Channel Steel Used for Machineryshows the peptides separations after enzymatic hydrolysis.Roller Hearth Heating Furnace for Gas Cylindrer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, but not shown inPP/Pet Woven Geotextile for Road Construction,custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, and also free amino acids (Whitaker et al., 1963).Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine,1996).

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CONCLUSIONS

China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts) for the financial support.

REFERENCES

Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM/ODM China Pet Meat Strip Making Machine -]

Brown, E. M; Thompson, C .J.,High Speed HDMI Cable Wholesale HDMI with Ethernet, M.M.(1994),Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Indoor Customized Electronic Lighted LED Neon Open Sign]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Women′s Suit in Reasonable Price. Analytical Biochemistry212,436-445[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

Dalev, P.G. and Simeonova, L.S.(1992),Pet Short Fiber Non Woven Geotextile for Landfill Filtration. Biotechnology letters 14,6, 531-534[Good Quality Snow White Pumpkin Seeds for Sale]

Heidemann, E.(1997),Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof.Leder 48,(5),99-104[China Factory Four Wire Spiral Hydraulic Hose DIN 856 En 4sh]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight]

Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Competitive Price Sodium Gluconate as Concrete Admixture.Precision Investment Casting Stainless Steel 304 Wire Rope Hook. USP/SP[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Leaf Shape Pearl Jewelry Wedding Tiara]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc Milling Machine Part]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cold Drawn D2 H13 Alloy Steel Turning Roll]

Custom Die Casting Aluminium Product for Connector Usage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Balustrade/Pillar/Column-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Metal Polishing Disc]

Custom Die Casting Aluminium Product for Connector Usage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Container House for Clinic or Hospital]

Custom Die Casting Aluminium Product for Connector Usage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, Part I.PP/Pet Woven Geotextile for Road Construction. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[20W Solar Street Light with Lens Motion Sensor 2 Years Warranty]

Lamella Clarifier in Industrial Wastewater Treatment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering.Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, 175-184[OEM Cast Ductile Grey Iron/Aluminum/Copper/Iron/Zinc/Stainless Steel for Automobile Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry]

Whitaker,J.R.(1963),Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights. Analytical Chemistry35(12), 1950-1953[300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Competitive Price Sodium Gluconate as Concrete AdmixtureAuthor for correspondence