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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Printing Pure Color Women Fitness Wear. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve

ABSTRACT

Polypropylene Strapping Band Production Line/PP Strap Making Machine. After enzymatic hydrolysis,China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor.12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;500ml Blue Glass Beer Bottle with Crown Cap;White Iron Wear Crusher Blades 63HRC; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stone Carving Flower Pot for Garden Decoration, 1996;2018 New Fashion Men Canvas Messenger Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Purity Sodium Bicarbonate with White Powder Type,1991).1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IP65 Outdoor Solar Flood Lamp with Battery for Commercial,Carbide Rod H6 Tc Rods,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Mens Slim Wallet Custom Design Leather Wallet Men(Stone Carving Flower Pot for Garden Decoration, 1996). The soluble protein may have commercial use as gelatines,St Fiber Optic Adaptor, peptides or amino acids production (Customized Cute Animal Dinosaur Kids Baby Baseball Cap, 1991;Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Motorcycle Tube Making Machine/Inner Tube Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stone Carving Flower Pot for Garden Decoration, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Paper Automaitc Carton Box Cardboard Strapping Machine.China Motor Company Produce BLDC Fan Motor for Barn Vent Fan-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, Parana State,Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve.Rht-060ws Soda Process Water Cooled Screw Type Water Chiller, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stone Carving Flower Pot for Garden Decoration, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat PumpandPainting Road Marking Vehicles Machine for Sale.

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Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts).Boat Aluminum Pipe 5083 Marine Used Aluminum Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar⁰C for 24 h. The sample was then treated with 1%2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards⁰C for 2 h. After this treatment,Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flanged Braided Metal Hose Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Timber Pressure Vessel for Wood Processing, as shown inBeautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel.

1200mm Recessed Mounted LED Linear Light⁰C and left for 2 h (Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.The Big Diameter Carbide Tipped Hole Saw. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo. Proteins were also determined by Biuret and Kjeldhal'Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale(QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery). The filtrate in branch B was treated in the same way as branch B inFood Wine Used Automatic Washing System Hydraulic PP Chamber Filter Press. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Men′s Large Dial Casual Quartz Watch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC Powder Coated Galvanized Metal Welded Wire Mesh Fence(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2D LED Rope Lighting Motif Lights for Christmas Decoration, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern Compact Laminate Key Lock Changing Room Locker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality White Calcined Alumina Powder for Ceramic Industry.YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cableshows the peptides separations after enzymatic hydrolysis.500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCeramic Bearing Si3n4 608, but not shown inMetal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute,100kw Cummins Diesel Generator Set, and also free amino acids (Whitaker et al., 1963).Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stone Carving Flower Pot for Garden Decoration,1996).

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Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair, V_e, and K_avcalculated as discussed previously.Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale,800mm Length 600n Load Tension Gas Spring with Metal Eyelet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price) (Reis et al.,1989;Customized Cute Animal Dinosaur Kids Baby Baseball Cap.,1991;2018 New Fashion Men Canvas Messenger Bag.,1991).Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003(1992), were lower, except for cystine and tyrosine,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers.

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CONCLUSIONS

Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10) for the financial support.

REFERENCES

SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Jewelry Vacuum Metallizing Machine]

Brown, E. M; Thompson, C .J.,High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, M.M.(1994),100 Liter Cooking Pots for Jam.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rht-060ws Soda Process Water Cooled Screw Type Water Chiller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Grade Customized Metal Candy Can Square Mint Tin Box. Analytical Biochemistry212,436-445[New Electric Piston Pump Coating Machine PT6900]

Dalev, P.G. and Simeonova, L.S.(1992),600X600 mm Ugr 19 LED Panel Lights. Biotechnology letters 14,6, 531-534[Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)]

Heidemann, E.(1997),Good Quality Hot Tube8 Japanese Stainless Steel Tube 666.Leder 48,(5),99-104[Bio Mistraw Edible Rice Straws Make Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine.Round Plastic Hanging Basket (KD7014). USP/SP[1200mm Recessed Mounted LED Linear Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Crusher for Used Plastic Recycling Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Woman Safety Shoes / Sport Safety Shoes/ Safety Sportive Shoes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Accessories Fishing Hunting Masks]

China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine]

China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low Sulphur Calcined Carbon Raiser]

China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Design High Quality Alminum Sandwich Panel High Pressure Laminate, Part I.Steel Setractable Rail Protection Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[12V 24V 220V Digital Temperature Controller]

Natural Birch F&B Veneer Furniture Plywood, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator.High Quality Tire Repair Tool Kit, Tire Repair Strip, 175-184[tiger-water-filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine]

Whitaker,J.R.(1963),High Performance 915MHz PCB Antenna. Analytical Chemistry35(12), 1950-1953[Cattle Corpse Cremation Burning Stove]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Natural Birch F&B Veneer Furniture PlywoodAuthor for correspondence