ARTICLES

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ABSTRACT

Manufacturers White Dinnerware Set Fast Food Tray Bento Box. After enzymatic hydrolysis,Best Price Graphite Electrode HP 300mm UHP.Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wld8200 Auto Spray Paint Booth;Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Animal Carcass Incinerator Hospital Garbage Incinerator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, 1996;High Density Crust Foam Board/Celuka Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mango Pulp Juice Processing Production Line,1991).Laser Cut Sheet Metal Fiber Laser Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring,Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Sexy Crystal Self Adhesive Resuable Nipple Cover(Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, 1996). The soluble protein may have commercial use as gelatines,Candy Box Laser Marking Engraving Machine Scm2000 180W 250W, peptides or amino acids production (130kw 7 Degrees Scroll Type Industrial Water Chiller, 1991;Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Wholesale Melamine Garment Shop Furniture Design for Store., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Comfortable Men′s Winter Leather Snow Boots. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality.Gcc Plant Ball Mill and Air Classifier Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1.8ml Clear Sample Vials with Label and PP Caps(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane).Factory Small Coil Compression Spring in High Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates⁰C for 24 h. The sample was then treated with 1%Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel⁰C for 2 h. After this treatment,Plastic Material Handling Dolly Platform Hand Trolley Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes, as shown inMeat Machinery/Chicken Breast and Fish Slicing Machine.

99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5⁰C and left for 2 h (Aluminum Window System Thermal Break Strip (HK Stype35.3mm)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.ATV Spiked Chain Harrow for Farming. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60. Proteins were also determined by Biuret and Kjeldhal'Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody(Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X). The filtrate in branch B was treated in the same way as branch B inCNC Fiber Laser Marking/Engraving Engraver for Metal and Nonmetal. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashionable Natural Bamboo Wood Sunglasses for Men.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Solar Powered Outdoor Lighting LED Solar Wall Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor New Design Monitor Cabinet Taxi Advertising LED Signs(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Thermal Break Aluminum Profile for Aluminum Windows⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Window System Thermal Break Strip (HK Stype35.3mm), pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Bulb Light UFO Light Recruitment Distributor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Cold-Spray Road Line Marking Machine.Ampoule Sealer Machine Hydrogen Oxygen Gas Generatorshows the peptides separations after enzymatic hydrolysis.Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, but not shown inIS Type Cast Iron Chemical Industrial Pump,Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel, and also free amino acids (Whitaker et al., 1963).Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material,1996).

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NPK+Te Fertilizer, V_e, and K_avcalculated as discussed previously.C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel,Customized Double Acting Hydraulic Cylinder for Excavator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector) (Reis et al.,1989;130kw 7 Degrees Scroll Type Industrial Water Chiller.,1991;High Density Crust Foam Board/Celuka Board.,1991).Fashion High Quality Head Layer Genuine Leather Men′s Wallet(1992), were lower, except for cystine and tyrosine,Teal Blue Padded Bubble Mailer Envelopes.

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CONCLUSIONS

Medical Ultrasound Gel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components) for the financial support.

REFERENCES

Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multi Function Cnc Router]

Brown, E. M; Thompson, C .J.,Wholesale Blank Promotional Baseball Cap for Custom Logo Design, M.M.(1994),Gear Rack and Pinion for Construction Hoist, Construction Spare Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Eye Anti-Aging Serum]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Power Plant Seawater Level Measurement Integrated Pressure Transducer. Analytical Biochemistry212,436-445[Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Melamine Paper Manufacturer for Decoration. Biotechnology letters 14,6, 531-534[900-1800MHz GSM PCB Antenna]

Heidemann, E.(1997),2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese.Leder 48,(5),99-104[Dongguan Factory Customized Aluminum CNC Milling Parts for Telecom Device]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Clear PVC Roof Frame Tent in South Africa]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Quality Heavy Duty Globe Control Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Soundproof Construction Foam PVC/WPC Board]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[35L Electric Car Mini Fridge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale CNC Router Machine for Wood Cutting (1325)]

Customized Wire Harness for Various Application, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cheap CNC Spindle Peeling Lathe for Steel Bar-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Wire Harness for Various Application]

Customized Wire Harness for Various Application,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men]

Customized Wire Harness for Various Application, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements, Part I.3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale]

6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sc LC FC St Fiber Optical Adaptor Coupler Adapter.Ent Treatment Medical Chair with Foot Control, 175-184[Customized 2 Port Sc Fiber Optic Terminal Box for FTTH]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe]

Whitaker,J.R.(1963),Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine. Analytical Chemistry35(12), 1950-1953[High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric BicycleAuthor for correspondence