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ABSTRACT

100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price. After enzymatic hydrolysis,15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine.High Speed Steel Mill Roll with High Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, 1996;LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ,1991).Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Latest New Hho Engine Oil Carbon Remover,Wood Chips Rotary Dryer Sawdust Pellet Drying Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Delicate Handmade Round Natural Stone Chain Necklace(Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, 1996). The soluble protein may have commercial use as gelatines,Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, peptides or amino acids production (Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light, 1991;Embossed Aluminium/ Aluminum Household Foil 8011-O, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cross Hex Flange Screw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings.Full Color Indoor Outdoor LED Curtain TV Display-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Plastic Injection Mold Parts Square Interlocks Maker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pepper Peanut Sauce Colliod Miller Grinding Machine, Parana State,Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry.Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theArtnet Node Artnet to DMX ControllerandHot Selling Custom Sheet Metal Fabrication.

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12.5mva Transformer 12500kVA 33kv 35kv Power Transformer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Double Sides Clear White Frosted Acrylic Sheet).Electronic Foldable for Patient Treatment Ent Unit Chair(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Pushbutton Ultrasonic Spin Welding Machine⁰C for 24 h. The sample was then treated with 1%500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles⁰C for 2 h. After this treatment,Double Shaft Paper Cardboard Waste Plastic Shredder Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Desktop Single Wave Soldering Machine/Wave Soldering Machine, as shown inChina Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part.

Manufacturers White Dinnerware Set Fast Food Tray Bento Box⁰C and left for 2 h (Wrinkle Effect Crease UV Drying Machine UV Curing System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Price Graphite Electrode HP 300mm UHP. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1. Proteins were also determined by Biuret and Kjeldhal'Wld8200 Auto Spray Paint Booth(Rooftop HVAC Packaged Units (RTU) Amrta Manufacture). The filtrate in branch B was treated in the same way as branch B inRed Light For Nursery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Animal Carcass Incinerator Hospital Garbage Incinerator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRal9002 White Prepainted Galvanized Steel Coil Z275 for Building Material(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mango Pulp Juice Processing Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Laser Cut Sheet Metal Fiber Laser Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Wholesale Melamine Garment Shop Furniture Design for Store.Junior Backyard Training Practise Soccer Goal Netshows the peptides separations after enzymatic hydrolysis.Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar, but not shown inPaper Automaitc Carton Box Cardboard Strapping Machine,Wholesale Comfortable Men′s Winter Leather Snow Boots, and also free amino acids (Whitaker et al., 1963).Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor,1996).

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CONCLUSIONS

1.8ml Clear Sample Vials with Label and PP Caps. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sludge Oil Separation Centrifuge) for the financial support.

REFERENCES

Factory Small Coil Compression Spring in High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[CNC Hydraulic Shearing Machine Cutting Machine Blades]

Brown, E. M; Thompson, C .J.,Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates, M.M.(1994),Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Material Handling Dolly Platform Hand Trolley Truck. Analytical Biochemistry212,436-445[Round Hole Carbon Steel Pipe Perforated for Filter]

Dalev, P.G. and Simeonova, L.S.(1992),Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump. Biotechnology letters 14,6, 531-534[Fashionable Natural Bamboo Wood Sunglasses for Men]

Heidemann, E.(1997),Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes.Leder 48,(5),99-104[Hikins Manufacture 80g RO Membrane Filter Water Filter Cartridge for Homeuse RO SysteM]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Antique Craft Running Competitive Metal Medal of Souvenir]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy]

Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashionable Natural Bamboo Wood Sunglasses for Men-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel DIN7981 Pan Head Self Tapping Screw]

Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus]

Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Solar Powered Outdoor Lighting LED Solar Wall Light, Part I.Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Arrival Plush Animal Ride for Kids for Shopping Center]

9~12 Inch Mechanical Backing Plate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Thermal Break Aluminum Profile for Aluminum Windows.Aluminum Window System Thermal Break Strip (HK Stype35.3mm), 175-184[Poultry Slaughter Equipment Chicken Slaughter Line]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[No 55 Steel Wire Cross Type Razor Barbed Wire]

Whitaker,J.R.(1963),LED Bulb Light UFO Light Recruitment Distributor. Analytical Chemistry35(12), 1950-1953[Indoor Load Break Switch Isolating Switch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

STPP Sodium Tripolyphosphate for Detergent / Ceramic / FoodAuthor for correspondence