ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-KERONG Security Safe Combination Smart Key Card Cabinet Door Lock. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Cast Floating Ball Valve China Manufacturer (GAQ41F)

ABSTRACT

PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. After enzymatic hydrolysis,Stainless Steel Main Pipe Tee Joint for Railway Piping System.Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Promotion Snack Children School Lunch Bag Cooler Bag;Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Automatic Paper Lunch Food Box Carton Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Core Solid Copper Conductor Rg Coaxial Cable, 1996;Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building,1991).Baby Clothes Wear Fleece Baby Bunting Onesie Jacket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Life Size 170cm Silicone Sex Dolls Real Love Doll for Male,Reflective Sheeting Material for Licence Plate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industrial Grade Nitrous Oxide for Hot Sales(Single Core Solid Copper Conductor Rg Coaxial Cable, 1996). The soluble protein may have commercial use as gelatines,Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove, peptides or amino acids production (Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, 1991;Fire Alarm Detector 24VDC Reflective Beam Detector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Mini Bag Spices Small Salt Packing Packaging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-No 55 Steel Wire Cross Type Razor Barbed Wire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Core Solid Copper Conductor Rg Coaxial Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4V330-10 Air Compressor Solenoid Industrial Valve.Professional Various Standard Hot Sale Rotary Lab Single Round Punch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Waterproof PVDF Aluminum Curtain Wall Panel, Parana State,Cast Floating Ball Valve China Manufacturer (GAQ41F).Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Single Core Solid Copper Conductor Rg Coaxial Cable, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNew Design Pet ProductandHigh Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17.

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Round Modern Furniture Leisure Sofa for Promotion(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950).Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sany Hg3333m Climbing Placing Boom⁰C for 24 h. The sample was then treated with 1%Plastic Packaging Tray Vacuum Forming Machine⁰C for 2 h. After this treatment,Permanent Suspended Magnetic Separator for Belt Conveyor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Double Sides Clear White Frosted Acrylic Sheet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, as shown in1200X1000X810 Strong Collapsible Plastic Containers for Storage.

20W Solar Street Light with Lens Motion Sensor 2 Years Warranty⁰C and left for 2 h (Kt Board Acrylic CCD Camera CNC Knife Cutting Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Machined Power Transmission Shaft Part. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. Proteins were also determined by Biuret and Kjeldhal'Good Price Channel Steel Used for Machinery(Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile). The filtrate in branch B was treated in the same way as branch B inNetwork Cable Copper Standard SFTP/FTP CAT6 LAN Cable Computer Monitor Security. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Real Leather Wallet Mens Designer Leather Wallets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare PartsandFull Port Small Automatic Electronic Plumbing Ball Valve. Enzymatic (Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,)Cleanroom White PVC Dotted Anti-Static Gloves(sulfuric acid)High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail(Single Core Solid Copper Conductor Rg Coaxial Cable, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Marine Solas Adhesive Reflective Tape, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Marine Solas Adhesive Reflective Tape, flow rate of 0.1 mL/min., and l = 620 nm.

G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room.3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturershows the peptides separations after enzymatic hydrolysis.Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWaw 8 Ton Logistics Light Truck for Transportation, but not shown inSheet Metal Distribution Cabinet with High Quality,ASTM A240 304 316L No. 1 Stainless Steel Sheet, and also free amino acids (Whitaker et al., 1963).12V 24V 220V Digital Temperature Controller. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Single Core Solid Copper Conductor Rg Coaxial Cable,1996).

Stacking

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Rotor High Output Electric 120 Watt Motor, V_e, and K_avcalculated as discussed previously.Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby,Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Double Sides Clear White Frosted Acrylic Sheet) (Reis et al.,1989;Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap.,1991;Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use.,1991).Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine(1992), were lower, except for cystine and tyrosine,Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile.

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CONCLUSIONS

Rpl Straight Socket and Plugs Male Thread. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor) for the financial support.

REFERENCES

46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sunroom Used Double Tempered Glass Thermal Break Aluminum Casement Window and Door]

Brown, E. M; Thompson, C .J.,OEM Metal Stamping Stainless Steel Parts with CNC Machining, M.M.(1994),Ss Wafer Type Check Valve Dn32 Dn40 Dn50.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rooftop HVAC Packaged Units (RTU) Amrta Manufacture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Roller Skating Sneakers Shoes for Children (8083)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. Analytical Biochemistry212,436-445[Junior Backyard Training Practise Soccer Goal Net]

Dalev, P.G. and Simeonova, L.S.(1992),Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape. Biotechnology letters 14,6, 531-534[Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar]

Heidemann, E.(1997),8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera.Leder 48,(5),99-104[Karl Mayer Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Paper Automaitc Carton Box Cardboard Strapping Machine]

Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),WPC Wood Plastic Board Equipment Extruder Machine.Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS. USP/SP[Cast Steel Dual Plate Wafer Check Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Neoprene Elbow Support: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[16A/24A High Speed Wire Cable Braiding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[(316L 304L 316ln 310S 316ti 347H 1.4835 1.4845 1.4404 1.4301 1.4571) Stainless Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Toughened Glass Disc Suspension Insulator]

Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Produce Medical Machining Custom Metal Milling-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine]

Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa]

Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, Part I.Custom Metal Stamping Welding Spare Parts for Farming. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet]

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation.275ml Frosted Clear Glass Drinking Bottle for Juice, 175-184[Large Capacity Good Price Rotary Drum Dryer From Reliable Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Building Material Sound Heat Insulation Rock Wool Sandwich Panel]

Whitaker,J.R.(1963),EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price. Analytical Chemistry35(12), 1950-1953[Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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