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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Wire Harness for Various Application, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cheap CNC Spindle Peeling Lathe for Steel Bar, 1996;Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a,1991).6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sc LC FC St Fiber Optical Adaptor Coupler Adapter,Ent Treatment Medical Chair with Foot Control,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine(Cheap CNC Spindle Peeling Lathe for Steel Bar, 1996). The soluble protein may have commercial use as gelatines,New Leaf Shape Pearl Jewelry Wedding Tiara, peptides or amino acids production (Starter Motor 0001367034 12V 3KW 9T for Mercedes, 1991;New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;HPMC Hydroxy Propyl Methyl Cellulose Additives., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cheap CNC Spindle Peeling Lathe for Steel Bar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags.Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Design Pet Product, Climbing Willow Cat Scratcher Tree(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile).Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate⁰C for 24 h. The sample was then treated with 1%Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide⁰C for 2 h. After this treatment,Single and Multi Rope Wire Mine Hoist From China. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery, as shown inGenuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automotive Rubber Parts for Any Rubber Material. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDie Casting Zinc Alloy Badge Use for Car(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CREE Chip 24W 28W 30W LED Downlight⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Three Phase 35kv Current Transformer for Indoor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Cheap Price Disposable IV Infusion Set with Precise Filter) for the financial support.

REFERENCES

Microfiber Towel Car Drying Soft Towel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[round glass candle holder glass tealight jar with screw thread]

Brown, E. M; Thompson, C .J.,Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, M.M.(1994),High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Paper Automaitc Carton Box Cardboard Strapping Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food. Analytical Biochemistry212,436-445[Cast Steel Dual Plate Wafer Check Valve]

Dalev, P.G. and Simeonova, L.S.(1992),100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine. Biotechnology letters 14,6, 531-534[Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve]

Heidemann, E.(1997),New Styles Fashion Erotic Women Underwear Sexy Lingerie.Leder 48,(5),99-104[Laser Pipe Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[16A/24A High Speed Wire Cable Braiding Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Scourer Spool Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet]

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Fully Automatic Water Bottle Lids Labeling Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold]

Fully Automatic Water Bottle Lids Labeling Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera, Part I.Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Round Hole Carbon Steel Pipe Perforated for Filter]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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