ARTICLES

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ABSTRACT

Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. After enzymatic hydrolysis,5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle.Clear PVC Roof Frame Tent in South Africa. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511);Stainless Steel Bend Pipe for Industrial Use; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ybz-E16f20 Vane Steering Hydraulic Pumps, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality Snow White Pumpkin Seeds for Sale, 1996;ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automotive Acrylic Wholesale Masking Tape Factory Price,1991).Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper,Watermrk Drop in Bathroom Basin Sink,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM ABS Industrial Plastic Sheet Injection Auto Part(Good Quality Snow White Pumpkin Seeds for Sale, 1996). The soluble protein may have commercial use as gelatines,15L Big Cooling Cylinder High Production Hard Ice Cream Machine, peptides or amino acids production (Flange Type Ball Float Water Tank Control Valve, 1991;925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Outdoor Plastic Chrome Auto Logo Signs.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Table CNC Marble Engraver for Stone. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality Snow White Pumpkin Seeds for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4.China Soundproof Construction Foam PVC/WPC Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Accessories Fishing Hunting Masks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cast Floating Ball Valve China Manufacturer (GAQ41F)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Main Pipe Tee Joint for Railway Piping System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

4V330-10 Air Compressor Solenoid Industrial Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gradient Print Overlay Push Button Membrane Keypad Switch) for the financial support.

REFERENCES

Professional Various Standard Hot Sale Rotary Lab Single Round Punch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Laser Cutting Steel Machine Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, M.M.(1994),Light Blue Color Glass Drink Bottle for Beer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Zs Round Vibrating Sieve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. Analytical Biochemistry212,436-445[ 20t]

Dalev, P.G. and Simeonova, L.S.(1992),IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. Biotechnology letters 14,6, 531-534[Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop]

Heidemann, E.(1997),Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.Leder 48,(5),99-104[Realtree Camouflage 3D Leafy Hat Hunting Face Mask]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[360 Degree Maverick Angler Fishing Kayak with Rotating Seat]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Playground Plastic Slide with Swing and Basketry]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Round Modern Furniture Leisure Sofa for Promotion: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Big Dimension UHP Graphite Electrodes for Steel Making]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bamboo Drying Machine High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Promotion Snack Children School Lunch Bag Cooler Bag]

Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sany Hg3333m Climbing Placing Boom-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Neodymium Magnet with RoHS ISO 16499 Approved]

Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Corrugated Carton Box Fully Automatic Folding Gluing Machine]

Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Packaging Tray Vacuum Forming Machine, Part I.Permanent Suspended Magnetic Separator for Belt Conveyor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Timber Pressure Vessel for Wood Processing]

Double Sides Clear White Frosted Acrylic Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery.20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, 175-184[High Lumen 100W LED Corn Light Apply to Warehouses High Bay Retrofit Bulb]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LED Outdoor Solar Lawn Garden Lighting for Wall]

Whitaker,J.R.(1963),CNC Machined Power Transmission Shaft Part. Analytical Chemistry35(12), 1950-1953[Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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