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ABSTRACT

19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS. After enzymatic hydrolysis,Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax.Dark Green Decorative Garden Welded Wire Mesh Fencing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, 1996;High Speed HDMI Cable Wholesale HDMI with Ethernet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine,1991).High Quality Women′s Suit in Reasonable Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pet Short Fiber Non Woven Geotextile for Landfill Filtration,Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Competitive Price Sodium Gluconate as Concrete Admixture(Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, 1996). The soluble protein may have commercial use as gelatines,Precision Investment Casting Stainless Steel 304 Wire Rope Hook, peptides or amino acids production (AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, 1991;Custom Die Casting Aluminium Product for Connector Usage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Balustrade/Pillar/Column., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PP/Pet Woven Geotextile for Road Construction. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lamella Clarifier in Industrial Wastewater Treatment.FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Sale CNC Router Machine for Wood Cutting (1325)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ltmg Classic Product 6 Ton Wheel Front Loader for Sale).Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%50g Body Luxury King Crown Cosmetic Containers⁰C for 24 h. The sample was then treated with 1%Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men⁰C for 2 h. After this treatment,Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Paper Egg Box Forming Molding Production Line/ Egg Tray Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine, as shown inChina Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib.

Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)⁰C and left for 2 h (275ml Frosted Clear Glass Drinking Bottle for Juice). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Metal Grinding Machine Tool Mk2120. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket. Proteins were also determined by Biuret and Kjeldhal'Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit(Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks). The filtrate in branch B was treated in the same way as branch B inNew Kind of Snake-Print True Leather Women′s Bag with European and American Fashion Cowhide Colour Platinum Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHydraulic Electric Stationary Car Platform Scissor Elevator Lift(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, pH 3.25., mobile phase B: buffer solution of 0.01 mol/N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Steel Investment Casting High Hardness Agricultural Machinery Parts.Householding High Precision Electrical Plastic Injection Parts/Mouldshows the peptides separations after enzymatic hydrolysis.Minimalist Slim Real Leather Front Pocket Card Wallet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant, but not shown in25kg Fertilizer Belt Packing Machine for Special Materials,Natural Cleaning Konjak Sponge, and also free amino acids (Whitaker et al., 1963).Indoor Load Break Switch Isolating Switch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl,1996).

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CONCLUSIONS

Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts) for the financial support.

REFERENCES

Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Forklift Agv Cart]

Brown, E. M; Thompson, C .J.,500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet, M.M.(1994),12 Inch King Mattress Latex Foam Memory Foam Mattress.The Journal of the American Leather Chemists Association (JALCA)89(7)[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook. Analytical Biochemistry212,436-445[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Dalev, P.G. and Simeonova, L.S.(1992),HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance. Biotechnology letters 14,6, 531-534[Tk 4100]

Heidemann, E.(1997),Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt.Leder 48,(5),99-104[PDA 1d 2D Upc/Pdf/Data Matrix/Maxicode/Qr Code Laser Bar Code Reader]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial P-Toluene Sulfonic Acid for 93%-99% Purity]

Hotel Card Door Lock Access Control Safe Door Lock, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable.Longyi Crimping Types Cable Terminal Lugs. USP/SP[Custom Grooved Quartz Plate Deep Processing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rigid PVC Foam Board for FRP Marine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Double Lane Inflatable Carnival Bungee Run Game]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Waterproof Anti-Slip XPE Foam Mat / Camping Mat / Beach Mat / Yoga Mat / Sleeping Ma T/ Kids Play Mat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle]

Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube]

Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W]

Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Furniture Gold PVD Plasma Coating Machine, Part I.Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap]

Steel Pipe Rust Removal Shot Blasting Cleaning Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hydraulic Press Corrugated Cold Roll Automatically Forming Machine.2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, 175-184[Samsung Excavator Parts Tcm Oil Seal Travel Motor Seal Kit Oil Seal Removal Tool Roten Mechanical Seal Excavator Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator]

Whitaker,J.R.(1963),Automatic Bread Crumb Processing Line and Making Extruder Machine. Analytical Chemistry35(12), 1950-1953[Professional Plastic Plating Makeup Brush Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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