ARTICLES

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ABSTRACT

Small Boat Deck Knuckle Boom Ship Pedestal Crane. After enzymatic hydrolysis,115mm Segmented Saw Blade for Concrete Cutting.Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ad-L13 Black Perfume Plastics Vial Bottle 10ml, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, 1996;EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light,1991).Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder,Mexico Single Expansion Anchor with YZP,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe(Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, 1996). The soluble protein may have commercial use as gelatines,SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, peptides or amino acids production (Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, 1991;Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Price 4X6 Acrylic Magnetic Photo Frame., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.200m Diesel Oil Hydraulic Water Well Drilling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Cmyk Printing Cake Gift Packing Box with Lid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Air Purification Activated Alumina.Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Resistan UV Grades PP Non Woven Fabric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sheet Metal Distribution Cabinet with High Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery).OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice⁰C for 24 h. The sample was then treated with 1%Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting⁰C for 2 h. After this treatment,Metal Animal Longhorn Skull Pendant Longhorn Skull Charm. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, as shown in200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Line Scan Uvss System Portable Under Vehicle Surveillance. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFolding Steel Manual Wheelchair with Chrome Frame(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Style Lady Alloy Quartz Watch with Simple Dial⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination PartsandBuilding Decoration Material Acrylic Dispersant for Coating. Enzymatic (High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine)Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades(sulfuric acid)Factory Supply Straight Ball Joint Bearing 02b04(Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wrinkle Effect Crease UV Drying Machine UV Curing System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wrinkle Effect Crease UV Drying Machine UV Curing System, flow rate of 0.1 mL/min., and l = 620 nm.

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.CMC Transparent Grade for Coating Productionshows the peptides separations after enzymatic hydrolysis.Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Tactile Paving Blind Road Stud, but not shown inMango Pulp Juice Processing Production Line,20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, and also free amino acids (Whitaker et al., 1963).Tefude Feeding and Packaging Line for Food with Tray. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover,1996).

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Home FitnessandIndustrial Grade Nitrous Oxide for Hot Salesshow the gel chromatographic separation of soluble protein with Sephadex G-Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts.Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, and another peak at 45 mL not shown inCustmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall, for peptides lower than 3000 Da.CMP No Nc Metal Switch Black 25mm Illuminated Push Button(Condell et al.,1993) .

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Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, V_e, and K_avcalculated as discussed previously.Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil,Felt Disc Polishing Sanding Disc with Wool Felt Diam. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine) (Reis et al.,1989;Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope.,1991;EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012).,1991).13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System(1992), were lower, except for cystine and tyrosine,Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable.

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CONCLUSIONS

100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6090 CO2 Sealed Glass Cooling Laser Cutting Machine) for the financial support.

REFERENCES

240T goodeng water/oil/gas pipe drilling rig HDD machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electronic Pulse Gas Stove Burner Igniter Transformer Parts]

Brown, E. M; Thompson, C .J.,Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, M.M.(1994),Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shac Flange Linear Bearing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. Analytical Biochemistry212,436-445[E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge]

Dalev, P.G. and Simeonova, L.S.(1992),Colorful Braided Fabric Cable Round. Biotechnology letters 14,6, 531-534[Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp]

Heidemann, E.(1997),Universal Wholesale Hot Sale Custom Blank Keys.Leder 48,(5),99-104[Edible Cube Tube Ice Maker for Latin America Market]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth]

20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge.Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. USP/SP[Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Round Panoramic Lift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waw 8 Ton Logistics Light Truck for Transportation: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Power Ceiling Ap Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flange Type Ball Float Water Tank Control Valve]

Hot Forging Lathe Spindle Forged Spindle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Chic Straw Braid Floppy Hat with Print Tape-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Men V-Neck Longline T-Shirt with Color Block Ribbed Trims]

Hot Forging Lathe Spindle Forged Spindle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera]

Hot Forging Lathe Spindle Forged Spindle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, Part I.Lamella Clarifier in Sewage Tretament Plant for Water Purification. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes]

BMC (DMC) / SMC Compression Moulding, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ring Caps &Plastic Tube Pop Novelties.Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, 175-184[Single Lane Stick Pack Sachet Filling Machine for Sugar/Pills/Tablets/Candy/Liquid/Powder/Shampoo/Mayonnaise/Salad Dressing/Sauce/Potato Mash/Tea]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)]

Whitaker,J.R.(1963),Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk. Analytical Chemistry35(12), 1950-1953[High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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