ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Spark Resistant Construction Axial Fan with Fixed Pitch Blade. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl

ABSTRACT

3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home. After enzymatic hydrolysis,25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25).Precision CNC Milling DIN69871. Mas403 for CNC Machinist. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, 1996;High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision Customized CNC Machined Mild Steel Flange for Automobile,1991).Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Metal Container Packaging for Edible Oil Tin Cans Storage Boxes,Melee Custom Plastic Mould New Design,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter(Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, 1996). The soluble protein may have commercial use as gelatines,Copper-Pressed V-Type Expansion Pipe Joint, peptides or amino acids production (Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber, 1991;White Desiccant Bag Silica Gel with Indicated Orange Bead, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Iron Wire Mesh Chain Link Mesh Security Fence., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter.Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:360 Degree Maverick Angler Fishing Kayak with Rotating Seat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (600X600 mm Ugr 19 LED Panel Lights).Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Crusher for Used Plastic Recycling Line⁰C for 24 h. The sample was then treated with 1%300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router⁰C for 2 h. After this treatment,Ceramic Bearing Si3n4 608. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Nbm High Quality Auto Part Factory Price 6pk2310's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay, as shown in8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts.

High Quality Mini LED Flood Light SMD Flood Light 50W⁰C and left for 2 h (Custom Free Logo Design Cheap Hand Flag (NF01F02021)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass. Proteins were also determined by Biuret and Kjeldhal'Professional Manufacture Metal Medal Promotional Gift(Cheap Wooden House Zinc Plated Angle Bracket). The filtrate in branch B was treated in the same way as branch B inKitchen Utensil Set Cooking Tools. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Singlemode Su Upc Fiber Optic Fast Connector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRoller Skating Sneakers Shoes for Children (8083)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric Heating Industrial Jacketed Glass Reactor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/40X-1600X Biological Microscope Xsz-107V LED Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/6063 Aluminum Coil for Commercial Vehicle Floor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stone Cracking Rock Cutting Cement.Fused Silica Quartz Lump 5-50mm for Refractory Materialsshows the peptides separations after enzymatic hydrolysis.8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, but not shown inWhite Iron Wear Crusher Blades 63HRC,PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards, and also free amino acids (Whitaker et al., 1963).Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box,1996).

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CONCLUSIONS

High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Big Bussiness Project Commerical Chicken Poultry Farm Buildings) for the financial support.

REFERENCES

Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[SMT Circuit Board Manufacturer Custom Electronic Assembly PCB PCBA]

Brown, E. M; Thompson, C .J.,Custom Good Quality Outdoor Music Wireless Cooler Speaker Box, M.M.(1994),OEM Viton Mechanical Rubber Oil Seal.The Journal of the American Leather Chemists Association (JALCA)89(7)[2018 New Fashion Men Canvas Messenger Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric. Analytical Biochemistry212,436-445[2D LED Rope Lighting Motif Lights for Christmas Decoration]

Dalev, P.G. and Simeonova, L.S.(1992),Q235 Q345 Hot Rolled Channel Steel. Biotechnology letters 14,6, 531-534[Colorful 100LED Copper String Light Outdoor LED]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Mini Bag Spices Small Salt Packing Packaging Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform]

Pure Water Cutting Head Assy for Waterjet Cutting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Deposited Stainless Steel Rivet Nut with Knurled]

Pure Water Cutting Head Assy for Waterjet Cutting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Resistan UV Grades PP Non Woven Fabric]

Pure Water Cutting Head Assy for Waterjet Cutting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Latest Lace Fabric Design Logo Flag Embroidery Patch, Part I.China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester]

High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05.New H Type Battery Cage System for Layer Chicken Farm, 175-184[Inside Groove Vernier Digital Calipers with Round Taper Point for Precision Measuring 4mm Stailess Steel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)]

Whitaker,J.R.(1963),Calcined Petroleum Coke as Best Recarburizer in Iron Industry. Analytical Chemistry35(12), 1950-1953[Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mini Pencil Tag with Lanyard (YS211)Author for correspondence