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^IICNPq/RHAE/biotechnology-Tefude Feeding and Packaging Line for Food with Tray. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts

ABSTRACT

Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. After enzymatic hydrolysis,CMP No Nc Metal Switch Black 25mm Illuminated Push Button.Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil;Felt Disc Polishing Sanding Disc with Wool Felt Diam; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, 1996;100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (240T goodeng water/oil/gas pipe drilling rig HDD machine,1991).Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength,Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Colorful Braided Fabric Cable Round(Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, 1996). The soluble protein may have commercial use as gelatines,Universal Wholesale Hot Sale Custom Blank Keys, peptides or amino acids production (Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, 1991;Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waw 8 Ton Logistics Light Truck for Transportation., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Forging Lathe Spindle Forged Spindle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Chic Straw Braid Floppy Hat with Print Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply.Lamella Clarifier in Sewage Tretament Plant for Water Purification-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:BMC (DMC) / SMC Compression Moulding(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Low Density Cleaner in Paper Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room).En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Stainless Steel Laser Cutting⁰C for 24 h. The sample was then treated with 1%Price of a Retail Supermarket Push Cart for Sale⁰C for 2 h. After this treatment,Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Handmade Natural Bamboo Basket / Bamboo Flower Pot, as shown inFlb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.

Stainless Steel Handrail Bar Holder for 16mm Pipe⁰C and left for 2 h (Pepper Peanut Sauce Colliod Miller Grinding Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Shac Flange Linear Bearing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. Proteins were also determined by Biuret and Kjeldhal'Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape(China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor). The filtrate in branch B was treated in the same way as branch B infor Smart Phone WiFi Video Recorder Watch&Sport Camera F26 Smart Watch Outdoor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Router for Engraving Cutting Carving Drilling Milling.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHorizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Hot Rolled Steel U Channel China Supplier⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/M Type Metallic Yarn Lurex Yarn Embroidery Yarn, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Corrugated Carton Box Fully Automatic Folding Gluing Machine.TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pumpshows the peptides separations after enzymatic hydrolysis.800lb 1/2 A105 Thread Forged Steel Globe Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPainting Road Marking Vehicles Machine for Sale, but not shown in Wire,Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, and also free amino acids (Whitaker et al., 1963).China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable,1996).

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CONCLUSIONS

2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Mobile Hydraulic Concrete Placing Boom, M.M.(1994),Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Supply Coaxial Cable Rg 59 with Messenger]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. Analytical Biochemistry212,436-445[350mm Diamond Blade for Granite]

Dalev, P.G. and Simeonova, L.S.(1992),Cr1000 Spray Nozzle Common Rail Injector Tester. Biotechnology letters 14,6, 531-534[Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck]

Heidemann, E.(1997),Industrial Cast Steel Flanged One Way Swing Check Valve Nrv.Leder 48,(5),99-104[Bedroom Dress Mirror]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Chic Straw Braid Floppy Hat with Print Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kt Board Acrylic CCD Camera CNC Knife Cutting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[NBR Latex Adhesive for Glove]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit]

High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rht-060ws Soda Process Water Cooled Screw Type Water Chiller]

High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Electric Piston Pump Coating Machine PT6900]

High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, Part I.Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

Whitaker,J.R.(1963),Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator. Analytical Chemistry35(12), 1950-1953[1200mm Recessed Mounted LED Linear Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial UseAuthor for correspondence