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ABSTRACT

3D Adjustable American Market Cabinet Door Mini Hinge. After enzymatic hydrolysis,Factory Sale Various Customized Square 6063 Extruded Aluminum Profile.Custom Design 100% Silk Tie for Men. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting;Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Om4 Sc/Upc-LC/Upc Fiber Optic Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Nylon Back to Back Hook&Loop, 1996;Canned Fruit Peach Processing Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate,1991).Garden Furniture Sofa Rattan Modular Corner Set with Cushions. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Water Cream Bulk Storage Tank,Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants(High Quality Nylon Back to Back Hook&Loop, 1996). The soluble protein may have commercial use as gelatines,Seasoned Cast Iron South Africa Three Legged Potjie Pot, peptides or amino acids production (1.0g Water and Air Ozone Generator with Silicone Tube, 1991;Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;16A/24A High Speed Wire Cable Braiding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Nylon Back to Back Hook&Loop, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰20 Inch 48V 750W Fat Tire Electric Bike with Double Seat.Agriculture Apple Orchard Reflective Pet Film-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3D Creative Design Metal Medal Promotion Gift of Sport, Parana State,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108).Ad-L13 Black Perfume Plastics Vial Bottle 10ml, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Nylon Back to Back Hook&Loop, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRoller Skating Sneakers Shoes for Children (8083)andJunior Backyard Training Practise Soccer Goal Net.

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Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Paper Automaitc Carton Box Cardboard Strapping Machine).EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light⁰C for 24 h. The sample was then treated with 1%Universal Mirco Forklift Car Bus Truck Seats Switch Sensors⁰C for 2 h. After this treatment,Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mexico Single Expansion Anchor with YZP's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe, as shown inCast Steel Dual Plate Wafer Check Valve.

SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet⁰C and left for 2 h (Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Whole Absorbtion Flexo Printing Soltting Die Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'Factory Price 4X6 Acrylic Magnetic Photo Frame(16A/24A High Speed Wire Cable Braiding Machine). The filtrate in branch B was treated in the same way as branch B inWholesale Strong Disc Shaped Neodymium Magnet N52 Custom Made. The final soluble protein shown were used for peptides separations using gel permeation chromatography,200m Diesel Oil Hydraulic Water Well Drilling Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Cmyk Printing Cake Gift Packing Box with Lid. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAir Purification Activated Alumina(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Resistan UV Grades PP Non Woven Fabric⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Scba Breathing Apparatus Equipment with Ce Certification, pH 3.25., mobile phase B: buffer solution of 0.01 mol/FRP Pultruded Pipe or GRP Profile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk.Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machineshows the peptides separations after enzymatic hydrolysis.New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHorizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, but not shown inReliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet,Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, and also free amino acids (Whitaker et al., 1963).Metal Animal Longhorn Skull Pendant Longhorn Skull Charm. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Nylon Back to Back Hook&Loop,1996).

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High Precision Mold Manufacturer Plastic Injection Mould, V_e, and K_avcalculated as discussed previously.China Wholesale Oxide Aluminium Black Sand Blast Media,Welded Stud Anchor Chain for Ship. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial) (Reis et al.,1989;1.0g Water and Air Ozone Generator with Silicone Tube.,1991;Canned Fruit Peach Processing Plant.,1991).SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry(1992), were lower, except for cystine and tyrosine,Line Scan Uvss System Portable Under Vehicle Surveillance.

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CONCLUSIONS

Folding Steel Manual Wheelchair with Chrome Frame. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit) for the financial support.

REFERENCES

Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling]

Brown, E. M; Thompson, C .J.,Fashion Style Lady Alloy Quartz Watch with Simple Dial, M.M.(1994),Rooftop HVAC Packaged Units (RTU) Amrta Manufacture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box. Analytical Biochemistry212,436-445[Stainless Steel DIN7981 Pan Head Self Tapping Screw]

Dalev, P.G. and Simeonova, L.S.(1992),Building Decoration Material Acrylic Dispersant for Coating. Biotechnology letters 14,6, 531-534[6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus]

Heidemann, E.(1997),High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine.Leder 48,(5),99-104[14 Pin Wire Harness Connector Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Arrival Plush Animal Ride for Kids for Shopping Center]

Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades.Factory Supply Straight Ball Joint Bearing 02b04. USP/SP[No 55 Steel Wire Cross Type Razor Barbed Wire]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Indoor Load Break Switch Isolating Switch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wrinkle Effect Crease UV Drying Machine UV Curing System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Price Arch Pipes Galvanized Steel Pipe Greenhouse Structure Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate]

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[EPS PS Foam Food Container Making Machine]

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Theme Park Inflatable Bouncer for Sale (BJ-B16)]

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, Part I.Tefude Feeding and Packaging Line for Food with Tray. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automotive Acrylic Wholesale Masking Tape Factory Price]

Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine.CMP No Nc Metal Switch Black 25mm Illuminated Push Button, 175-184[2019 Unisex Adults Black Twill Fabric with White Mesh and Embroidery Printed Logo Snapback Baseball Golf Cap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting]

Whitaker,J.R.(1963),Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. Analytical Chemistry35(12), 1950-1953[120ml Glass Perfume Bottles for Designer Perfume]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Polymeric Ferric Sulfate (SPFS/PFS) for Water TreatmentAuthor for correspondence