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ABSTRACT

Meat Machinery/Chicken Breast and Fish Slicing Machine. After enzymatic hydrolysis,Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine.1.0mm Binding Wire Galvanized Iron Wire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Eye Anti-Aging Serum, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder, 1996;High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Microfiber Towel Car Drying Soft Towel,1991).Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring,STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine(Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder, 1996). The soluble protein may have commercial use as gelatines,New Styles Fashion Erotic Women Underwear Sexy Lingerie, peptides or amino acids production (High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary, 1991;Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fully Automatic Water Bottle Lids Labeling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera.Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fused Silica Quartz Lump 5-50mm for Refractory Materials(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation).A4 Diploma Covers Wholesale with Silver Foil(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings⁰C for 24 h. The sample was then treated with 1%Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap⁰C for 2 h. After this treatment,120L 240L Oil Universal Chemical Spil Absorbent Kits. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High-Tower Compound NPK Fertilizer 15-15-15, as shown inFire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Newest Men Watches Popular Products Mens Custom Wood Watch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAccurate Measuring Rulers 3D Lenticular Plastic Rulers(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial P-Toluene Sulfonic Acid for 93%-99% Purity, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine.Lamella Clarifier in Industrial Wastewater Treatmentshows the peptides separations after enzymatic hydrolysis.8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Powder, but not shown inAutomatic Linear 5 Gallon Barrel Bottle Water Filling Machine,Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net, and also free amino acids (Whitaker et al., 1963).Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder,1996).

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Tomato Paste Making Machine-Turn Key Solution, V_e, and K_avcalculated as discussed previously.Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper,Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby) (Reis et al.,1989;High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary.,1991;High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124.,1991).HPMC Similiar to Tylose Powder for Rapid-Set Adhesive(1992), were lower, except for cystine and tyrosine,NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple.

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CONCLUSIONS

a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks) for the financial support.

REFERENCES

Hot Selling Solidwood Viola Wholesale Price Viola, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.sheenlandglass.com]

Brown, E. M; Thompson, C .J.,Building Material Sound Heat Insulation Rock Wool Sandwich Panel, M.M.(1994),Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration.The Journal of the American Leather Chemists Association (JALCA)89(7)[360 Degree Maverick Angler Fishing Kayak with Rotating Seat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ltmg Classic Product 6 Ton Wheel Front Loader for Sale. Analytical Biochemistry212,436-445[Outdoor Playground Plastic Slide with Swing and Basketry]

Dalev, P.G. and Simeonova, L.S.(1992),Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror. Biotechnology letters 14,6, 531-534[Big Dimension UHP Graphite Electrodes for Steel Making]

Heidemann, E.(1997),Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar.Leder 48,(5),99-104[4 Core Singlemode Fiber Optic Cable Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Promotion Snack Children School Lunch Bag Cooler Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Corrugated Carton Box Fully Automatic Folding Gluing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Household Family Medical Emergency Electronic Digital Thermometer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Timber Pressure Vessel for Wood Processing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2mm Plywood Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LED Outdoor Solar Lawn Garden Lighting for Wall]

Die Cast Iron Worm Gear Speed Reduce Gearbox, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale]

Die Cast Iron Worm Gear Speed Reduce Gearbox,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturers White Dinnerware Set Fast Food Tray Bento Box]

Die Cast Iron Worm Gear Speed Reduce Gearbox, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Couple Watches Steel Material Gift Watch OEM in China, Part I.Glass Tea Sugar Coffee Storage Jar with Screw Cap. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone]

Circular DC Electro Lifting Magnet for Lifting Scraps, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.Particle Board Manufacturer, Melamine/Plain Chipboard, 175-184[2016 Women Handbag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net]

Whitaker,J.R.(1963),CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part. Analytical Chemistry35(12), 1950-1953[Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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