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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Minimalist Slim Real Leather Front Pocket Card Wallet. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Natural Cleaning Konjak Sponge

ABSTRACT

Indoor Load Break Switch Isolating Switch. After enzymatic hydrolysis,Biodegradable Disposible Straw Pulp Food Box Ppp-1007.T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment;NBR Latex Adhesive for Glove; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cheap Wooden House Zinc Plated Angle Bracket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, 1996;Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw,1991).Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer,500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,12 Inch King Mattress Latex Foam Memory Foam Mattress(4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, 1996). The soluble protein may have commercial use as gelatines,A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, peptides or amino acids production (HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, 1991;Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Longyi Crimping Types Cable Terminal Lugs.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rigid PVC Foam Board for FRP Marine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display.41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Furniture Gold PVD Plasma Coating Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic Bread Crumb Processing Line and Making Extruder Machine, Parana State,Natural Cleaning Konjak Sponge.Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Blank Promotional Baseball Cap for Custom Logo DesignandIP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor.

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High Tensisle Strength Tissue Paper⁰C and left for 2 h (Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tractor Mounted Beach Cleaning Equipment Large Size. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap. Proteins were also determined by Biuret and Kjeldhal'Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts(Silicon Carbide Foundry Porous Ceramic Foam Filter). The filtrate in branch B was treated in the same way as branch B inTray Type Hot Air Circulating Food Drying Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PP Coated Pipe Machine Extruder Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSuper Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi Column Load Cell 100kg, 20t, pH 3.25., mobile phase B: buffer solution of 0.01 mol/LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

X15 Power Monitor Two Way Live Stage Monitor Speaker SystemandMarine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck. Enzymatic (Foldable PP Plastic Storage Logistics Turnover Box)China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005(sulfuric acid)Buddhist Mood Picture Wallpaper Murals(4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Flange Dismantling Joint, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Flange Dismantling Joint, flow rate of 0.1 mL/min., and l = 620 nm.

Duplex Stainless Steel Floating Ball Valve.Light Blue Color Glass Drink Bottle for Beershows the peptides separations after enzymatic hydrolysis.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, but not shown in Retail Shop Glass Display,Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, and also free amino acids (Whitaker et al., 1963).Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head,1996).

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CONCLUSIONS

Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hydraulic Press Corrugated Cold Roll Automatically Forming Machine) for the financial support.

REFERENCES

Fluorous Rubber Coated Fiberglass Cloth, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[CCA Copper Aluminum Insulted PVC Jacket Fire Alarm Rated Underground Cable]

Brown, E. M; Thompson, C .J.,Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, M.M.(1994),280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern).The Journal of the American Leather Chemists Association (JALCA)89(7)[200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Power Consumption Silicone Sex Doll Vending Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PS Foam Disposable Fast Food Box Making Machine. Analytical Biochemistry212,436-445[China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price]

Dalev, P.G. and Simeonova, L.S.(1992),K Helical Bevel Gearboxes Reducer Motor. Biotechnology letters 14,6, 531-534[Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts]

Heidemann, E.(1997),Indoor Customized Electronic Lighted LED Neon Open Sign.Leder 48,(5),99-104[Non Asbestos Rubber for Gasket O Ring Seal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CMC Transparent Grade for Coating Production]

Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar.Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. USP/SP[Stainless Steel Tactile Paving Blind Road Stud]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mango Pulp Juice Processing Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Free Logo Design Cheap Hand Flag (NF01F02021): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gym Exercise Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Home Fitness]

125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial Grade Nitrous Oxide for Hot Sales]

125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall]

125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:RJ45 Cat5e in-Line Coupler Modular Jack, Part I.Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tractor Mounted Beach Cleaning Equipment Large Size]

Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052.High Quality Disposable Blood Transfusion Set with Filter, 175-184[10 Degree Moving Walk]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine]

Whitaker,J.R.(1963),DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle. Analytical Chemistry35(12), 1950-1953[Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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