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ABSTRACT

Candy Box Laser Marking Engraving Machine Scm2000 180W 250W. After enzymatic hydrolysis,130kw 7 Degrees Scroll Type Industrial Water Chiller.Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Comfortable Men′s Winter Leather Snow Boots, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality, 1996;Gcc Plant Ball Mill and Air Classifier Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes,1991).Outdoor Playground Plastic Slide with Swing and Basketry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat,Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Belt Conveyor Systmem for Fertilizer Production Line(Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality, 1996). The soluble protein may have commercial use as gelatines,Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price, peptides or amino acids production (Digger Spring for Excavator Machinery, 1991;1.8ml Clear Sample Vials with Label and PP Caps, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Small Coil Compression Spring in High Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Material Handling Dolly Platform Hand Trolley Truck.Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5Alcalase2.5 L DX,ATV Spiked Chain Harrow for Farming, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Fashionable Natural Bamboo Wood Sunglasses for Men, Parana State,New Sexy Crystal Self Adhesive Resuable Nipple Cover.Solar Powered Outdoor Lighting LED Solar Wall Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLatest Lace Fabric Design Logo Flag Embroidery Patchand15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi.

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Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Beans).9~12 Inch Mechanical Backing Plate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Thermal Break Aluminum Profile for Aluminum Windows⁰C for 24 h. The sample was then treated with 1%Aluminum Window System Thermal Break Strip (HK Stype35.3mm)⁰C for 2 h. After this treatment,LED Bulb Light UFO Light Recruitment Distributor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ozone Generator Plant for Water Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator, as shown inWhole Absorbtion Flexo Printing Soltting Die Cutting Machine.

Free Assembly, Walking Machine, Home Fitness, Treadmill⁰C and left for 2 h (Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CMC Transparent Grade for Coating Production. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine. Proteins were also determined by Biuret and Kjeldhal'Cold-Spray Road Line Marking Machine(Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer). The filtrate in branch B was treated in the same way as branch B inMini CNC Glass Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rock Tunnel Boring Machine for Natural Gas Pipeline, pH 3.25., mobile phase B: buffer solution of 0.01 mol/NPK+Te Fertilizer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light.Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bagsshows the peptides separations after enzymatic hydrolysis.Wholesale Blank Promotional Baseball Cap for Custom Logo Design(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCNC Produce Medical Machining Custom Metal Milling, but not shown inAuto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05,Gear Rack and Pinion for Construction Hoist, Construction Spare Parts, and also free amino acids (Whitaker et al., 1963).Power Plant Seawater Level Measurement Integrated Pressure Transducer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality,1996).

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Tungsten Solid Carbide Straight 4/6 Flutes Reamer, V_e, and K_avcalculated as discussed previously.China Quality Heavy Duty Globe Control Valve,Customized Wire Harness for Various Application. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0) (Reis et al.,1989;Digger Spring for Excavator Machinery.,1991;Gcc Plant Ball Mill and Air Classifier Production Line.,1991).Cheap CNC Spindle Peeling Lathe for Steel Bar(1992), were lower, except for cystine and tyrosine,Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements.

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60) for the financial support.

REFERENCES

6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electric Fuel Pump]

Brown, E. M; Thompson, C .J.,Sc LC FC St Fiber Optical Adaptor Coupler Adapter, M.M.(1994),Ent Treatment Medical Chair with Foot Control.The Journal of the American Leather Chemists Association (JALCA)89(7)[1.0g Water and Air Ozone Generator with Silicone Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Wholesale Oxide Aluminium Black Sand Blast Media]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine. Analytical Biochemistry212,436-445[Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror]

Dalev, P.G. and Simeonova, L.S.(1992),New Leaf Shape Pearl Jewelry Wedding Tiara. Biotechnology letters 14,6, 531-534[CNC Router for Engraving Cutting Carving Drilling Milling]

Heidemann, E.(1997),Starter Motor 0001367034 12V 3KW 9T for Mercedes.Leder 48,(5),99-104[10ml Roll on Metal Ball Perfume Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Brand New Forklift with Double Front pneumatic Tire]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Furniture Gold PVD Plasma Coating Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Water-Proof Nylon Cable Conduit Glands]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Mens Slim Wallet Custom Design Leather Wallet Men]

Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Commercial Inflatable Dry Slide for Kids]

Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Regular Size Cigarette Rolling Papers]

Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging, Part I.Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Melamine/Plain Chipboard]

Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Water Chiller Air Cooled for Cooling Beer Refrigerating.High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, 175-184[Mobile Machinery Air Oil Heat Exchanger]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert]

Whitaker,J.R.(1963),Q3210 Vibratory Polishing Shot Blasting Machine. Analytical Chemistry35(12), 1950-1953[Painted G80 Cargo Lashing Chain with Hooks]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

GRP/FRP Profile Structures/Fiberglass Pultrusion ProductAuthor for correspondence