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ABSTRACT

Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer. After enzymatic hydrolysis,Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane.16oz Insulated Cardboad Cup Sleeve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wood Fireplace Double Skin Chimney Flue Pipes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality 304 Stainless Steel Sheet for Home Decoration Material, 1996;T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LED Rectangular Recessed Linear Lamps for Bank and Car Sale,1991).Salon Equipment Hair Clipper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Flat Conveyor Belt Steel Idler Frame for Roller,LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle(High Quality 304 Stainless Steel Sheet for Home Decoration Material, 1996). The soluble protein may have commercial use as gelatines,Durable TPR Pet Molar Toy with Colorful Rope, peptides or amino acids production (Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, 1991;Chromium Oxide Green 99% for Construction and Plastics, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Zs Round Vibrating Sieve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Tactile Paving Blind Road Stud.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-70L Lab Use Refrigerated Circulator Chiller Factory Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality 304 Stainless Steel Sheet for Home Decoration Material, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500).Roller Hearth Heating Furnace for Gas Cylindrer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:custom design MIFARE DESFire EV2 2K plastic RFID loyalty card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dark Green Decorative Garden Welded Wire Mesh Fencing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0).Csm DIY Microscope for Kids/Toy Microscope(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Oak Veneer Laminated Plywood for Decoration⁰C for 24 h. The sample was then treated with 1%China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib⁰C for 2 h. After this treatment,Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Speed HDMI Cable Wholesale HDMI with Ethernet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, as shown inRemovable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Die Casting Aluminium Product for Connector Usage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PP/Pet Woven Geotextile for Road Construction, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lamella Clarifier in Industrial Wastewater Treatment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Wholesale Oxide Aluminium Black Sand Blast MediaandFPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. Enzymatic (Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED)Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights(sulfuric acid)Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick(High Quality 304 Stainless Steel Sheet for Home Decoration Material, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, flow rate of 0.1 mL/min., and l = 620 nm.

IS Type Cast Iron Chemical Industrial Pump.Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirrorshows the peptides separations after enzymatic hydrolysis.Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCNC Router for Engraving Cutting Carving Drilling Milling, but not shown inPopular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair,Custom Grooved Quartz Plate Deep Processing, and also free amino acids (Whitaker et al., 1963).Hot Sale CNC Router Machine for Wood Cutting (1325). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality 304 Stainless Steel Sheet for Home Decoration Material,1996).

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CONCLUSIONS

Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer) for the financial support.

REFERENCES

Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Air Driven Diaphragm Pump]

Brown, E. M; Thompson, C .J.,Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM, M.M.(1994),12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Painted G80 Cargo Lashing Chain with Hooks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[GRP/FRP Profile Structures/Fiberglass Pultrusion Product]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hydraulic Electric Stationary Car Platform Scissor Elevator Lift. Analytical Biochemistry212,436-445[Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. Biotechnology letters 14,6, 531-534[Ozone Generator Plant for Water Treatment]

Heidemann, E.(1997),Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal.Leder 48,(5),99-104[New Products P2.81 LED Screen LED Video Wall Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Scented Flavor Flower Herbal Tea]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 49]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zt-18 Open-Type Cable Drag Chain]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller]

Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quaity China Supplier Truck Trailer Auto Car Accessories-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Factory Light Soda Ash /Sodium Carbonate]

Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery]

Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable, Part I.Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Csm DIY Microscope for Kids/Toy Microscope]

Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Minimalist Slim Real Leather Front Pocket Card Wallet.Natural Cleaning Konjak Sponge, 175-184[m.hybonsecurity-eas.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)]

Whitaker,J.R.(1963),Indoor Load Break Switch Isolating Switch. Analytical Chemistry35(12), 1950-1953[High Quality Electronic Circuit Board Supplier PCB Assembly]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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