ARTICLES

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ABSTRACT

Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts. After enzymatic hydrolysis,Household Family Medical Emergency Electronic Digital Thermometer.Die Cast Iron Worm Gear Speed Reduce Gearbox. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Glass Tea Sugar Coffee Storage Jar with Screw Cap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Circular DC Electro Lifting Magnet for Lifting Scraps, 1996;Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Particle Board Manufacturer, Melamine/Plain Chipboard,1991).CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G),Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps(Circular DC Electro Lifting Magnet for Lifting Scraps, 1996). The soluble protein may have commercial use as gelatines,120ml Glass Perfume Bottles for Designer Perfume, peptides or amino acids production (Agriculture Hay Silage Wrapping Film, 1991;Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.16oz Insulated Cardboad Cup Sleeve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Outdoor Simple Gazebo Beer Festival Marquee Tent. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Circular DC Electro Lifting Magnet for Lifting Scraps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HOWO Tipper Truck Price with Manufacture Factory Price.Wood Fireplace Double Skin Chimney Flue Pipes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 304 Stainless Steel Sheet for Home Decoration Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Durable TPR Pet Molar Toy with Colorful Rope(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles).Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chromium Oxide Green 99% for Construction and Plastics⁰C for 24 h. The sample was then treated with 1%Zs Round Vibrating Sieve⁰C for 2 h. After this treatment,Stainless Steel Tactile Paving Blind Road Stud. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)70L Lab Use Refrigerated Circulator Chiller Factory Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), as shown inDC 12V 24V Mini Portable Car Refrigerator Compressor Fridge.

Roller Hearth Heating Furnace for Gas Cylindrer⁰C and left for 2 h (Neoprene Elbow Support). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.custom design MIFARE DESFire EV2 2K plastic RFID loyalty card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016. Proteins were also determined by Biuret and Kjeldhal'Hotel Card Door Lock Access Control Safe Door Lock(High Quality 304 Stainless Steel Sheet for Home Decoration Material). The filtrate in branch B was treated in the same way as branch B inStainless Steel Spani Puri Frying Machine Chin Chin Frying Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel DIN7981 Pan Head Self Tapping Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dark Green Decorative Garden Welded Wire Mesh Fencing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Csm DIY Microscope for Kids/Toy Microscope, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Oak Veneer Laminated Plywood for Decoration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pet Short Fiber Non Woven Geotextile for Landfill Filtration.Professional Stainless Steel Biological Fermentorshows the peptides separations after enzymatic hydrolysis.Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFemo Ferro Molybdenum Powder Lump 55/60, but not shown inL Shaped Modular Kitchen Designs Kitchen Cabinet on Sale,Competitive Price Sodium Gluconate as Concrete Admixture, and also free amino acids (Whitaker et al., 1963).Precision Investment Casting Stainless Steel 304 Wire Rope Hook. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Circular DC Electro Lifting Magnet for Lifting Scraps,1996).

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3-Pin Australia Power Cord Plug with SAAandEcofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jarshow the gel chromatographic separation of soluble protein with Sephadex G-AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.Custom Die Casting Aluminium Product for Connector Usage, and another peak at 45 mL not shown inHydraulic Electric Stationary Car Platform Scissor Elevator Lift, for peptides lower than 3000 Da.Stainless Steel Balustrade/Pillar/Column(Condell et al.,1993) .

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Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, V_e, and K_avcalculated as discussed previously.PP/Pet Woven Geotextile for Road Construction,Lamella Clarifier in Industrial Wastewater Treatment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice) (Reis et al.,1989;Agriculture Hay Silage Wrapping Film.,1991;Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.,1991).FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering(1992), were lower, except for cystine and tyrosine,Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED.

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CONCLUSIONS

Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Salon Equipment Hair Clipper) for the financial support.

REFERENCES

Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[SGS Approved PC Polycarbonate Hollow Sheet Solid Sheet UV Coated Corrugated Sheet]

Brown, E. M; Thompson, C .J.,Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, M.M.(1994),IS Type Cast Iron Chemical Industrial Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine. Analytical Biochemistry212,436-445[2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Grooved Quartz Plate Deep Processing. Biotechnology letters 14,6, 531-534[Design Luxury Moon Cake Box with Hot Stamp Logo]

Heidemann, E.(1997),Hot Sale CNC Router Machine for Wood Cutting (1325).Leder 48,(5),99-104[m.baiyipackage.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Minimalist Slim Real Leather Front Pocket Card Wallet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Air Purification Activated Alumina]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ce Certificate Fast Food Box Production Line (MT105/120)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sany Hg3333m Climbing Placing Boom]

Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Paper Egg Box Forming Molding Production Line/ Egg Tray Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Teal Blue Padded Bubble Mailer Envelopes]

Mini Succulent Planters, Cactus Pots with Wooden Cement Pot,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge]

Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine, Part I.Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box]

CNC Metal Grinding Machine Tool Mk2120, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket.Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit, 175-184[4.75mm/4.8mm Power Plug Pin]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners]

Whitaker,J.R.(1963),Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM. Analytical Chemistry35(12), 1950-1953[Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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