ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Foldable PP Plastic Storage Logistics Turnover Box. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005

ABSTRACT

Buddhist Mood Picture Wallpaper Murals. After enzymatic hydrolysis,High Quality Flange Dismantling Joint.Duplex Stainless Steel Floating Ball Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card;Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, 1996;CNC Machine Aluminum Telescopic Covers Flat Bellows Cover., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input,1991).Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kubota Combine Harvester Spare Parts 5h484-2392 Nut,Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor(Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, 1996). The soluble protein may have commercial use as gelatines,Steel Floor Deck Post Cutting Cold Roll Forming Machine, peptides or amino acids production (Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), 1991;Fluorous Rubber Coated Fiberglass Cloth, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PS Foam Disposable Fast Food Box Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰K Helical Bevel Gearboxes Reducer Motor.Indoor Customized Electronic Lighted LED Neon Open Sign-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

RJ45 Cat5e in-Line Coupler Modular Jack, Parana State,China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005.Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEmbossed Aluminium/ Aluminum Household Foil 8011-OandLamella Clarifier in Sewage Tretament Plant for Water Purification.

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Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder).Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Disposable Blood Transfusion Set with Filter⁰C for 24 h. The sample was then treated with 1%DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle⁰C for 2 h. After this treatment,Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Brushless DC Electric Motor for Industrial Deduster's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Factory Light Soda Ash /Sodium Carbonate, as shown inGasoline Rototiller.

Regular Size Cigarette Rolling Papers⁰C and left for 2 h (China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.304L Stainless Steel U Channel Good Performance. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4). Proteins were also determined by Biuret and Kjeldhal'Junior Backyard Training Practise Soccer Goal Net(Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair). The filtrate in branch B was treated in the same way as branch B inHv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. The final soluble protein shown were used for peptides separations using gel permeation chromatography,350mm Diamond Blade for Granite.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Welding Stainless Steel Sheet Metal Welding Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUnbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Arrival Plush Animal Ride for Kids for Shopping Center⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal.Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouseshows the peptides separations after enzymatic hydrolysis.1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTc/Sc/Tb/Sb Oil Seal Much Stock on Sale, but not shown inPrefabricated Steel Structure Beam Frame Factory Offer Commercial Building,New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, and also free amino acids (Whitaker et al., 1963).Green Color PVC Anti Dazzle Panel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt,1996).

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CONCLUSIONS

Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card) for the financial support.

REFERENCES

Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Floor Scrubber Pads]

Brown, E. M; Thompson, C .J.,Hot Sale 840 Type PPGI Color Steel Roofing Sheets, M.M.(1994),FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[25kg Fertilizer Belt Packing Machine for Special Materials]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CATV Indoor Optical Receiver FTTH. Analytical Biochemistry212,436-445[Jc680 High Temperature Pressure Transmitter]

Dalev, P.G. and Simeonova, L.S.(1992),Best Sell 16L Square Dustbin Frame Mould. Biotechnology letters 14,6, 531-534[Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment]

Heidemann, E.(1997),Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover.Leder 48,(5),99-104[6090 CO2 Sealed Glass Cooling Laser Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Thermal Break Aluminum Profile for Aluminum Windows]

Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush.Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml. USP/SP[En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Insulation Top Hung Aluminum Awning Window with Mosquito Net]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PVDF Exterior Design Curved Aluminum Panel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Marine Solas Adhesive Reflective Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Disposable Photophobic Precision IV Set/Infusion Set with Best Quality]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing]

Neodymium Magnet with RoHS ISO 16499 Approved, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products]

Neodymium Magnet with RoHS ISO 16499 Approved,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

Neodymium Magnet with RoHS ISO 16499 Approved, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, Part I.High Pulling Force Rotatable Rare Earth Crane Magnet Hook. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women.High-Performance Thermal Break Profiles Aluminium for Doors and Windows, 175-184[crazyanimemerchandise]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Whitaker,J.R.(1963),Design Luxury Moon Cake Box with Hot Stamp Logo. Analytical Chemistry35(12), 1950-1953[Tk 4100]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial P-Toluene Sulfonic Acid for 93%-99% PurityAuthor for correspondence