ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Wholesale Promotion Snack Children School Lunch Bag Cooler Bag. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b

ABSTRACT

Full Automatic Paper Lunch Food Box Carton Forming Machine. After enzymatic hydrolysis,Single Core Solid Copper Conductor Rg Coaxial Cable.Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building;Baby Clothes Wear Fleece Baby Bunting Onesie Jacket; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Reflective Sheeting Material for Licence Plate, 1996;Industrial Grade Nitrous Oxide for Hot Sales., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove,1991).Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fire Alarm Detector 24VDC Reflective Beam Detector,Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Mini Bag Spices Small Salt Packing Packaging Machine(Reflective Sheeting Material for Licence Plate, 1996). The soluble protein may have commercial use as gelatines,No 55 Steel Wire Cross Type Razor Barbed Wire, peptides or amino acids production (4V330-10 Air Compressor Solenoid Industrial Valve, 1991;Professional Various Standard Hot Sale Rotary Lab Single Round Punch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Light Blue Color Glass Drink Bottle for Beer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Reflective Sheeting Material for Licence Plate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproof PVDF Aluminum Curtain Wall Panel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Plastic Packaging Tray Vacuum Forming Machine, Parana State,Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b.Permanent Suspended Magnetic Separator for Belt Conveyor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Reflective Sheeting Material for Licence Plate, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro JapaneseandCixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place.

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Double Sides Clear White Frosted Acrylic Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (16 Spindle Lace High Speed Braiding Machine Textile Machinery).5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%20W Solar Street Light with Lens Motion Sensor 2 Years Warranty⁰C for 24 h. The sample was then treated with 1%CNC Machined Power Transmission Shaft Part⁰C for 2 h. After this treatment,Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Price Channel Steel Used for Machinery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, as shown inHoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01).

Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology⁰C and left for 2 h (CH Series Pid Intelligent Temperature Controller CH102). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Direct Sale Patent up to Down Rubber Tile Curing Press. Proteins were also determined by Biuret and Kjeldhal'Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China(16oz Insulated Cardboad Cup Sleeve). The filtrate in branch B was treated in the same way as branch B inEverexceed 36V50A Pengiun SCR Single or Three Phase Motive / Intelligent/Industrial / Microprocessor / Forklift Battery Charger;. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Real Leather Wallet Mens Designer Leather Wallets.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Port Small Automatic Electronic Plumbing Ball Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cleanroom White PVC Dotted Anti-Static Gloves⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Marine Solas Adhesive Reflective Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm WhiteandG01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room. Enzymatic (Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers)ASTM A240 304 316L No. 1 Stainless Steel Sheet(sulfuric acid)12V 24V 220V Digital Temperature Controller(Reflective Sheeting Material for Licence Plate, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, flow rate of 0.1 mL/min., and l = 620 nm.

25kg Fertilizer Belt Packing Machine for Special Materials.6000 Series Anodizing Extrusion Aluminum Tubeshows the peptides separations after enzymatic hydrolysis.Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Efficiency Scc638 Water-Based Cutting Fluid with Factory Production, but not shown inProfessional Manufacture Metal Medal Promotional Gift,Rotor High Output Electric 120 Watt Motor, and also free amino acids (Whitaker et al., 1963).Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Reflective Sheeting Material for Licence Plate,1996).

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Rpl Straight Socket and Plugs Male Thread, V_e, and K_avcalculated as discussed previously.46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall,OEM Metal Stamping Stainless Steel Parts with CNC Machining. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket) (Reis et al.,1989;4V330-10 Air Compressor Solenoid Industrial Valve.,1991;Industrial Grade Nitrous Oxide for Hot Sales.,1991).Ss Wafer Type Check Valve Dn32 Dn40 Dn50(1992), were lower, except for cystine and tyrosine,2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe.

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CONCLUSIONS

Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket) for the financial support.

REFERENCES

8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Glass Water Pictcher Glass Water Jug Glass Drink Jug Glass Beverage Jug Glass Pitcher]

Brown, E. M; Thompson, C .J.,WPC Wood Plastic Board Equipment Extruder Machine, M.M.(1994),Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS.The Journal of the American Leather Chemists Association (JALCA)89(7)[12 Inch King Mattress Latex Foam Memory Foam Mattress]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Double Door Heated Holding Carts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Neoprene Elbow Support. Analytical Biochemistry212,436-445[Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates]

Dalev, P.G. and Simeonova, L.S.(1992),Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules. Biotechnology letters 14,6, 531-534[Aluminum Hot Forging Accessories Machining Parts]

Heidemann, E.(1997),CNC Produce Medical Machining Custom Metal Milling.Leder 48,(5),99-104[Salt and Pepper Mills Novel Salt & Pepper & Spice Grinders Red White Black Electric Pepper Mill Creative Kitchen Tools]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection]

Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Multi Sensor Nilitary Surveillance Thermal Imaging Security camera.Custom Metal Stamping Welding Spare Parts for Farming. USP/SP[Gear Rack and Pinion for Construction Hoist]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Density Crust Foam Board/Celuka Board]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[240T goodeng water/oil/gas pipe drilling rig HDD machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic Floor For Tennis Court]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine]

Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),275ml Frosted Clear Glass Drinking Bottle for Juice-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Flower Embroidery Applique Patches]

Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting]

Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price, Part I.Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Outdoor Plastic Chrome Auto Logo Signs]

Bolts and Nuts Set Brass Fasteners Double Hex Bolt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Direct Sales Kids Boy Cotton Children T Shirt.Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener, 175-184[Hotel Shower Handle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting]

Whitaker,J.R.(1963),15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. Analytical Chemistry35(12), 1950-1953[Q235 Q345 Hot Rolled Channel Steel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2Author for correspondence