ARTICLES

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ABSTRACT

Multi Sensor Nilitary Surveillance Thermal Imaging Security camera. After enzymatic hydrolysis,Custom Metal Stamping Welding Spare Parts for Farming.Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, 1996;Bolts and Nuts Set Brass Fasteners Double Hex Bolt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Direct Sales Kids Boy Cotton Children T Shirt,1991).Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi,Light Blue PVC Plastic Sheet and PVC Film,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy(Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, 1996). The soluble protein may have commercial use as gelatines,Metric Female Cone Hydraulic Hose Fitting, peptides or amino acids production (Hot Sale Dental Material Dental PMMA Block From China, 1991;Plastic Waste Pet Flakes Recycling Washing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Deposited Stainless Steel Rivet Nut with Knurled., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Stainless Steel Mechanical Filter Hosing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Paper New Year Greeting Cards Wedding Invitations Cards. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01).BLE5.0 Ttl to Bluetooth Module with at Command-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sludge Oil Separation Centrifuge).Easy Foldable Kids Walking Chair Toys Baby Walker(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Men V-Neck Longline T-Shirt with Color Block Ribbed Trims⁰C for 24 h. The sample was then treated with 1%Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener⁰C for 2 h. After this treatment,Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Multifunctional Solar Power Flashlight (SYSG-517)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product, as shown inHappy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post.

Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass⁰C and left for 2 h (Real Leather Wallet Mens Designer Leather Wallets). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FC Bare Fiber Optic Adapter for Network. Proteins were also determined by Biuret and Kjeldhal'3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory(OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk). The filtrate in branch B was treated in the same way as branch B inHigh Quality Univeral 1 DIN Car CD/DVD Player with USB/SD/Aux. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cattle Corpse Cremation Burning Stove.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avE26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Stainless Steel Biological Fermentor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rj11, Rj12, RJ45 Network Cable Modular Plug Crimping Tool Lk-Nt005, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum.Stone Cracking Rock Cutting Cementshows the peptides separations after enzymatic hydrolysis.900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge, but not shown inChina Manufacturer of Venlo PC or Venlo Glass Greenhouse,Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe, and also free amino acids (Whitaker et al., 1963).Plastic Flange Housing Ucf310-115 for Electric Hand Tools. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs,1996).

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Tct Circular Saw Blade &Diamond Saw Blade, V_e, and K_avcalculated as discussed previously.Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable,Lifting Thread with Needle for Plastic Surgery. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1) (Reis et al.,1989;Hot Sale Dental Material Dental PMMA Block From China.,1991;Bolts and Nuts Set Brass Fasteners Double Hex Bolt.,1991).Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls(1992), were lower, except for cystine and tyrosine,LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car.

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CONCLUSIONS

Fudan Compatible Card 1k RFID Card 13.56MHz. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving) for the financial support.

REFERENCES

Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Designer Pendant Light]

Brown, E. M; Thompson, C .J.,Biodegradable Food Packaging Take out Food Boxes, M.M.(1994),Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Wire]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations. Analytical Biochemistry212,436-445[Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel]

Dalev, P.G. and Simeonova, L.S.(1992),AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring. Biotechnology letters 14,6, 531-534[Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector]

Heidemann, E.(1997),20 Ton Small Vertical Plastic Injection Molding Machine.Leder 48,(5),99-104[19mm 1no Black Momentary Flat Metal Push Button Switch with Screw Terminal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5.5X19mm Hex Head Self Tapping Screws with Washer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ceramic Bearing Si3n4 608]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Codos Hair Clippers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute]

Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Supply Coaxial Cable Rg 59 with Messenger]

Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle]

Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine, Part I.Professional Plastic Plating Makeup Brush Set. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[350mm Diamond Blade for Granite]

Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine.Road Construction Non Woven Needle Punched PP Non Woven Geotextile, 175-184[Plastic Button Wood Engraving CO2 Laser Marking Machine Price 60W 80W CO2 Glass Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck]

Whitaker,J.R.(1963),Wholesale Scented Flavor Flower Herbal Tea. Analytical Chemistry35(12), 1950-1953[EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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