ARTICLES

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ABSTRACT

Tefude Feeding and Packaging Line for Food with Tray. After enzymatic hydrolysis,Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts.Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Felt Disc Polishing Sanding Disc with Wool Felt Diam, 1996;13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable,1991).100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,240T goodeng water/oil/gas pipe drilling rig HDD machine,Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength(Felt Disc Polishing Sanding Disc with Wool Felt Diam, 1996). The soluble protein may have commercial use as gelatines,Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen, peptides or amino acids production (Colorful Braided Fabric Cable Round, 1991;Universal Wholesale Hot Sale Custom Blank Keys, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waw 8 Ton Logistics Light Truck for Transportation. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Felt Disc Polishing Sanding Disc with Wool Felt Diam, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Forging Lathe Spindle Forged Spindle.Hot Sale Chic Straw Braid Floppy Hat with Print Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, Parana State,20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory.Dual Plastic 9 Volt Battery Clips Connector with Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Felt Disc Polishing Sanding Disc with Wool Felt Diam, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bagand2PC Flanged&Threaded.

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Precision Ball Screw with Two Nut(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover).EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low Density Cleaner in Paper Making Machine⁰C for 24 h. The sample was then treated with 1%En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada⁰C for 2 h. After this treatment,Customized Stainless Steel Laser Cutting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Price of a Retail Supermarket Push Cart for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, as shown inFlanged Braided Metal Hose Connector.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Router for Engraving Cutting Carving Drilling Milling(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Hot Rolled Steel U Channel China Supplier, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Low Sulphur Calcined Carbon Raiser, V_e, and K_avcalculated as discussed previously.Hot Selling Free Sample Natural Fragrant Bath SPA Salt,Custom FSTN Va COB Cog Segment LCD Display Module. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Microfiber Towel Car Drying Soft Towel) (Reis et al.,1989;Colorful Braided Fabric Cable Round.,1991;13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System.,1991).Jc680 High Temperature Pressure Transmitter(1992), were lower, except for cystine and tyrosine,Deep Hole Cylinder Boring Machine for Sale.

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CONCLUSIONS

Hot Selling 202 Stainless Steel Coil. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ring Caps &Plastic Tube Pop Novelties) for the financial support.

REFERENCES

NPK 16X Hammer Spare Parts Racing Forged Piston, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5ml 10ml Disposable Pipette]

Brown, E. M; Thompson, C .J.,2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, M.M.(1994),Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[White Iron Wear Crusher Blades 63HRC]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anti-Pollution Modified Acrylic Solid Surface for Countertops]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mobile Hydraulic Concrete Placing Boom. Analytical Biochemistry212,436-445[Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector]

Dalev, P.G. and Simeonova, L.S.(1992),Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700. Biotechnology letters 14,6, 531-534[Gcc Plant Ball Mill and Air Classifier Production Line]

Heidemann, E.(1997),Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll.Leder 48,(5),99-104[High Quality Customized Small Lotion Plastic Bottle Cap Flip Top Bottle Cap]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Watermrk Drop in Bathroom Basin Sink]

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cr1000 Spray Nozzle Common Rail Injector Tester.Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. USP/SP[Pure Water Cutting Head Assy for Waterjet Cutting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Black White Stone 925 Silver Cross Pendants Necklace Jewelry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Shipping To Shanghai]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags]

Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Kt Board Acrylic CCD Camera CNC Knife Cutting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2018 New Fashion Men Canvas Messenger Bag]

Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card]

Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, Part I.Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2D LED Rope Lighting Motif Lights for Christmas Decoration]

Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3.China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part, 175-184[How To Design A Perfume Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Colorful 100LED Copper String Light Outdoor LED]

Whitaker,J.R.(1963),Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box. Analytical Chemistry35(12), 1950-1953[Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Metal Cuting CNC Plasma Cutting Machine (DW1325)Author for correspondence