ARTICLES

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ABSTRACT

Duplex Stainless Steel Floating Ball Valve. After enzymatic hydrolysis,E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card.Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, 1996;Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kubota Combine Harvester Spare Parts 5h484-2392 Nut,1991).Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor,Steel Floor Deck Post Cutting Cold Roll Forming Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)(7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, 1996). The soluble protein may have commercial use as gelatines,Fluorous Rubber Coated Fiberglass Cloth, peptides or amino acids production (Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, 1991;280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PS Foam Disposable Fast Food Box Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.K Helical Bevel Gearboxes Reducer Motor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Indoor Customized Electronic Lighted LED Neon Open Sign. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar.Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Free Logo Design Cheap Hand Flag (NF01F02021)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Disposable Blood Transfusion Set with Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Accurate Measuring Rulers 3D Lenticular Plastic Rulers).DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)⁰C for 24 h. The sample was then treated with 1%High Quality Brushless DC Electric Motor for Industrial Deduster⁰C for 2 h. After this treatment,China Factory Light Soda Ash /Sodium Carbonate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Regular Size Cigarette Rolling Papers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)304L Stainless Steel U Channel Good Performance, as shown inWholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag.

Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)⁰C and left for 2 h (2PC Flanged&Threaded). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Junior Backyard Training Practise Soccer Goal Net. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,350mm Diamond Blade for Granite. Proteins were also determined by Biuret and Kjeldhal'Custom Welding Stainless Steel Sheet Metal Welding Parts(Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover). The filtrate in branch B was treated in the same way as branch B in80ml / 100ml / 120ml Skin Care Acrylic Plastic Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Laser ID Card Rigid PET Printing Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Arrival Plush Animal Ride for Kids for Shopping Center(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ss316 Stainless Steel Heavy Duty Straps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar.Flanged Braided Metal Hose Connectorshows the peptides separations after enzymatic hydrolysis.Green Color PVC Anti Dazzle Panel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inThree-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, but not shown inUniversal Wholesale Hot Sale Custom Blank Keys,ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, and also free amino acids (Whitaker et al., 1963).XL-21 Motive Electrical Switchgear Power Cabinet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input,1996).

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CONCLUSIONS

Hot Sale 840 Type PPGI Color Steel Roofing Sheets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (RJ45 Cat5e in-Line Coupler Modular Jack) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,CATV Indoor Optical Receiver FTTH, M.M.(1994),Best Sell 16L Square Dustbin Frame Mould.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Wooden House Zinc Plated Angle Bracket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Microfiber Towel Car Drying Soft Towel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover. Analytical Biochemistry212,436-445[Fused Silica Quartz Lump 5-50mm for Refractory Materials]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush. Biotechnology letters 14,6, 531-534[Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps]

Heidemann, E.(1997),Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml.Leder 48,(5),99-104[80mic High Bonding Double Sided Tissue Adhesive Tape (DOH08)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[White Iron Wear Crusher Blades 63HRC]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gcc Plant Ball Mill and Air Classifier Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hand Push Manual Rice Planter Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Watermrk Drop in Bathroom Basin Sink]

Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Pulling Force Rotatable Rare Earth Crane Magnet Hook-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pure Water Cutting Head Assy for Waterjet Cutting Machine]

Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter]

Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products, Part I.Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag]

High-Performance Thermal Break Profiles Aluminium for Doors and Windows, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Design Luxury Moon Cake Box with Hot Stamp Logo.Standard Industrial Double Nut Ball Screw Dfu Series, 175-184[Japanese Komatsu S6d110 Piston Ring Diesel Engine Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags]

Whitaker,J.R.(1963),Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter. Analytical Chemistry35(12), 1950-1953[2018 New Fashion Men Canvas Messenger Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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