ARTICLES

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ABSTRACT

CNC Metal Grinding Machine Tool Mk2120. After enzymatic hydrolysis,Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket.Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM;12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hydraulic Electric Stationary Car Platform Scissor Elevator Lift, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, 1996;Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo,1991).N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste),Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quaity China Supplier Truck Trailer Auto Car Accessories(Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, 1996). The soluble protein may have commercial use as gelatines,Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable, peptides or amino acids production (Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, 1991;Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Minimalist Slim Real Leather Front Pocket Card Wallet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Natural Cleaning Konjak Sponge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Indoor Load Break Switch Isolating Switch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Biodegradable Disposible Straw Pulp Food Box Ppp-1007.T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards).500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12 Inch King Mattress Latex Foam Memory Foam Mattress⁰C for 24 h. The sample was then treated with 1%A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook⁰C for 2 h. After this treatment,HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable, as shown inCold Drawn D2 H13 Alloy Steel Turning Roll.

Longyi Crimping Types Cable Terminal Lugs⁰C and left for 2 h ( Metal Polishing Disc). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rigid PVC Foam Board for FRP Marine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display. Proteins were also determined by Biuret and Kjeldhal'41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)( Container House for Clinic or Hospital). The filtrate in branch B was treated in the same way as branch B inFashion Hair Ornaments Pearl Jewellery Bridal Tiaras Crown. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Furniture Gold PVD Plasma Coating Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSteel Pipe Rust Removal Shot Blasting Cleaning Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Bread Crumb Processing Line and Making Extruder Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

20W Solar Street Light with Lens Motion Sensor 2 Years WarrantyandChina Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring. Enzymatic (300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve)Plastic Injection Molding Companies China for Tablet PC Case(sulfuric acid)27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools(Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Solid Tungsten Carbide Rods, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Solid Tungsten Carbide Rods, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head) for the financial support.

REFERENCES

Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Remote Control Tint Smart Glass]

Brown, E. M; Thompson, C .J.,Foldable PP Plastic Storage Logistics Turnover Box, M.M.(1994),China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Machine Aluminum Telescopic Covers Flat Bellows Cover]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Buddhist Mood Picture Wallpaper Murals. Analytical Biochemistry212,436-445[Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Flange Dismantling Joint. Biotechnology letters 14,6, 531-534[Factory Price 4X6 Acrylic Magnetic Photo Frame]

Heidemann, E.(1997),Duplex Stainless Steel Floating Ball Valve.Leder 48,(5),99-104[factory low price Extruding Granulation Machine - Vertical Fertilizer Chain Crusher - Exceed ]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax]

Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card.Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. USP/SP[Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wld8200 Auto Spray Paint Booth]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Biodegradable Disposible Straw Pulp Food Box Ppp-1007]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Commercial Rubber Floor Tiles Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[NPK 16X Hammer Spare Parts Racing Forged Piston]

Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Machine Aluminum Telescopic Covers Flat Bellows Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder]

Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4V330-10 Air Compressor Solenoid Industrial Valve]

Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, Part I.Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1 Ton Toyo Type Manual Chain Block]

Kubota Combine Harvester Spare Parts 5h484-2392 Nut, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable.HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor, 175-184[China Storage Batteries]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer]

Whitaker,J.R.(1963),Steel Floor Deck Post Cutting Cold Roll Forming Machine. Analytical Chemistry35(12), 1950-1953[Price of a Retail Supermarket Push Cart for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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