ARTICLES

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ABSTRACT

China 1 & 100 RMB Acrylic Currence Frame. After enzymatic hydrolysis,1 Ton Toyo Type Manual Chain Block.Spark Resistant Construction Axial Fan with Fixed Pitch Blade. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Precision CNC Milling DIN69871. Mas403 for CNC Machinist, 1996;Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Building Material of Granite Ceramic Wall Tile for Wall Decoration,1991).3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box,High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Precision Customized CNC Machined Mild Steel Flange for Automobile(Precision CNC Milling DIN69871. Mas403 for CNC Machinist, 1996). The soluble protein may have commercial use as gelatines,Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing, peptides or amino acids production (Metal Container Packaging for Edible Oil Tin Cans Storage Boxes, 1991;Melee Custom Plastic Mould New Design, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Copper-Pressed V-Type Expansion Pipe Joint.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Precision CNC Milling DIN69871. Mas403 for CNC Machinist, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰White Desiccant Bag Silica Gel with Indicated Orange Bead.High Quality Iron Wire Mesh Chain Link Mesh Security Fence-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Big Bussiness Project Commerical Chicken Poultry Farm Buildings(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Crusher for Used Plastic Recycling Line).400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20⁰C for 24 h. The sample was then treated with 1%New Electric Piston Pump Coating Machine PT6900⁰C for 2 h. After this treatment,200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Crusher for Used Plastic Recycling Line, as shown inOutdoor Accessories Fishing Hunting Masks.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCreative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Singlemode Su Upc Fiber Optic Fast Connector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Roller Skating Sneakers Shoes for Children (8083), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Metal Cuting CNC Plasma Cutting Machine (DW1325). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera) for the financial support.

REFERENCES

Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Workout Tracksuit Men′s Gym Wear 4 Pieces Suit Clothes]

Brown, E. M; Thompson, C .J.,Expanded Metal Infill Panel Metal Mesh Custom Made, M.M.(1994),DC Motor for Massagers.The Journal of the American Leather Chemists Association (JALCA)89(7)[5.5X19mm Hex Head Self Tapping Screws with Washer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Customization Screen Printing Machine for Porous Ceramic]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage. Analytical Biochemistry212,436-445[High Quaity China Supplier Truck Trailer Auto Car Accessories]

Dalev, P.G. and Simeonova, L.S.(1992),Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry. Biotechnology letters 14,6, 531-534[FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering]

Heidemann, E.(1997),Custom Good Quality Outdoor Music Wireless Cooler Speaker Box.Leder 48,(5),99-104[Propylene Glycol CAS No.: 57-55-6 Industrial Grade 99.5% & Pharmaceutical Grade 99.8%/99.9% USP]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Exporter of Wheat Flour Mill Plant (80t)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Q235 Q345 Hot Rolled Channel Steel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Simple Style European Design White Ceramic Square Plate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory]

OEM Factory New Girls Nuts Crunch Cheap Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hsac Shac Linear Guideway-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wood Chips Rotary Dryer Sawdust Pellet Drying Machine]

OEM Factory New Girls Nuts Crunch Cheap Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence]

OEM Factory New Girls Nuts Crunch Cheap Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, Part I.Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Beech Wood Chiavari Chair at a Competitive]

Pure Water Cutting Head Assy for Waterjet Cutting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat.Latest Lace Fabric Design Logo Flag Embroidery Patch, 175-184[12V 30ah LiFePO4 Lithium-Ion Iron Phosphate Car Batteries]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gradient Print Overlay Push Button Membrane Keypad Switch]

Whitaker,J.R.(1963),China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken. Analytical Chemistry35(12), 1950-1953[Glass Tea Sugar Coffee Storage Jar with Screw Cap]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Teflon Seal/Seals Spring EnergizedAuthor for correspondence