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ABSTRACT

1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine. After enzymatic hydrolysis,New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar.Green Color PVC Anti Dazzle Panel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Furniture Grade E1 Glue Plywood with Competitive Price, 1996;High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (TUV Certificate High Quality Single Core PV1-F PV Solar Cables,1991).Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cast Steel Dual Plate Wafer Check Valve,Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts(Furniture Grade E1 Glue Plywood with Competitive Price, 1996). The soluble protein may have commercial use as gelatines,Hot Sale 840 Type PPGI Color Steel Roofing Sheets, peptides or amino acids production (FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, 1991;CATV Indoor Optical Receiver FTTH, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Sell 16L Square Dustbin Frame Mould., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Furniture Grade E1 Glue Plywood with Competitive Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml.PVDF Exterior Design Curved Aluminum Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Neodymium Magnet with RoHS ISO 16499 Approved(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Design Luxury Moon Cake Box with Hot Stamp Logo(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electronic Foldable for Patient Treatment Ent Unit Chair).Standard Industrial Double Nut Ball Screw Dfu Series(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter⁰C for 24 h. The sample was then treated with 1%Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card⁰C for 2 h. After this treatment,Customized Logo Outdoor Hanging Swinging Camping Hammock. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning, as shown inStainless Steel Balustrade/Pillar/Column.

Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h⁰C and left for 2 h (Custom Die Casting Aluminium Product for Connector Usage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Metal Detector for Gold, Silver, etc Underground Metel Detector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hing Quality Rubber O Ring in Motor High Pressure. Proteins were also determined by Biuret and Kjeldhal'Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe(Foska Mini Size High Quality Heavy Duty Stapler). The filtrate in branch B was treated in the same way as branch B in12mm Vandal Push Button White Led. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMultifunctional Luxury Double SL 3D Zero Gravity Massage Chair(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Free Shipping Wholesale Peruvian Human Hair Weave, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, V_e, and K_avcalculated as discussed previously.IP65 Outdoor Solar Flood Lamp with Battery for Commercial,Carbide Rod H6 Tc Rods. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary) (Reis et al.,1989;FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector.,1991;High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn.,1991).High Quality Mens Slim Wallet Custom Design Leather Wallet Men(1992), were lower, except for cystine and tyrosine,St Fiber Optic Adaptor.

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CONCLUSIONS

Customized Cute Animal Dinosaur Kids Baby Baseball Cap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Pulling Force Rotatable Rare Earth Crane Magnet Hook) for the financial support.

REFERENCES

Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.etonsmtauto.com]

Brown, E. M; Thompson, C .J.,Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording, M.M.(1994),Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover.The Journal of the American Leather Chemists Association (JALCA)89(7)[Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Square Shape Glass Candle Holder for One Piece Tealight Candle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Motorcycle Tube Making Machine/Inner Tube Making Machine. Analytical Biochemistry212,436-445[Hot Selling Solidwood Viola Wholesale Price Viola]

Dalev, P.G. and Simeonova, L.S.(1992),Paper Automaitc Carton Box Cardboard Strapping Machine. Biotechnology letters 14,6, 531-534[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Heidemann, E.(1997),China Motor Company Produce BLDC Fan Motor for Barn Vent Fan.Leder 48,(5),99-104[Inflatable Air Sofa PVC Flocked Armchair for Kids]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB.1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank. USP/SP[Full Port Small Automatic Electronic Plumbing Ball Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20 Ton Small Vertical Plastic Injection Molding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[The Big Diameter Carbide Tipped Hole Saw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Castings Truck Wheel Hub]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[NPK+Te Fertilizer]

New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, Part I.Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cold-Spray Road Line Marking Machine]

Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Boat Aluminum Pipe 5083 Marine Used Aluminum Tube.Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, 175-184[Diamond Blade Korea]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Portable Powerful Charger with Dual Pd Charger and Type-C Charger]

Whitaker,J.R.(1963),2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards. Analytical Chemistry35(12), 1950-1953[Singlemode Su Upc Fiber Optic Fast Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Motor Company Produce BLDC Fan Motor for Barn Vent FanAuthor for correspondence