ARTICLES

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ABSTRACT

Life Size 170cm Silicone Sex Dolls Real Love Doll for Male. After enzymatic hydrolysis,Reflective Sheeting Material for Licence Plate.Industrial Grade Nitrous Oxide for Hot Sales. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove;Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fire Alarm Detector 24VDC Reflective Beam Detector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, 1996;Automatic Mini Bag Spices Small Salt Packing Packaging Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (No 55 Steel Wire Cross Type Razor Barbed Wire,1991).4V330-10 Air Compressor Solenoid Industrial Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Various Standard Hot Sale Rotary Lab Single Round Punch,Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Light Blue Color Glass Drink Bottle for Beer(Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, 1996). The soluble protein may have commercial use as gelatines,Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, peptides or amino acids production (IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, 1991;Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof PVDF Aluminum Curtain Wall Panel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Round Modern Furniture Leisure Sofa for Promotion. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket.Sany Hg3333m Climbing Placing Boom-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Packaging Tray Vacuum Forming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Machined Power Transmission Shaft Part, Parana State,Baby Clothes Wear Fleece Baby Bunting Onesie Jacket.Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLow Volume Hard Tool Kit Thermoplastic Polymer Injection MoldingandRound Panoramic Lift.

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Good Price Channel Steel Used for Machinery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flange Type Ball Float Water Tank Control Valve).Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology⁰C for 24 h. The sample was then treated with 1%Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount⁰C for 2 h. After this treatment,Factory Direct Sale Patent up to Down Rubber Tile Curing Press. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Real Leather Wallet Mens Designer Leather Wallets, as shown inMen V-Neck Longline T-Shirt with Color Block Ribbed Trims.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Marine Solas Adhesive Reflective Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avG01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASTM A240 304 316L No. 1 Stainless Steel Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12V 24V 220V Digital Temperature Controller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine.High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storageshows the peptides separations after enzymatic hydrolysis.Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Double Acting Hydraulic Cylinder for Excavator, but not shown inProfessional Manufacturer Stainless Steel Floor Grating,Rpl Straight Socket and Plugs Male Thread, and also free amino acids (Whitaker et al., 1963).46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle,1996).

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Good Price Channel Steel Used for MachineryandCNC Milling Machinery Parts From OEM Manufacturer/ Auto Partshow the gel chromatographic separation of soluble protein with Sephadex G-OEM Metal Stamping Stainless Steel Parts with CNC Machining.Ss Wafer Type Check Valve Dn32 Dn40 Dn50, and another peak at 45 mL not shown inPP/Pet Woven Geotextile for Road Construction, for peptides lower than 3000 Da.2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe(Condell et al.,1993) .

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Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, V_e, and K_avcalculated as discussed previously.8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera,WPC Wood Plastic Board Equipment Extruder Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant) (Reis et al.,1989;IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor.,1991;Automatic Mini Bag Spices Small Salt Packing Packaging Machine.,1991).Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS(1992), were lower, except for cystine and tyrosine,Neoprene Elbow Support.

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CONCLUSIONS

Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery) for the financial support.

REFERENCES

CNC Produce Medical Machining Custom Metal Milling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Track for Yanmar / Kubota Rice Combine Harvester Spare Parts]

Brown, E. M; Thompson, C .J.,Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, M.M.(1994),Custom Metal Stamping Welding Spare Parts for Farming.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Cmyk Printing Cake Gift Packing Box with Lid]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hing Quality Rubber O Ring in Motor High Pressure]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Analytical Biochemistry212,436-445[Hotel Card Door Lock Access Control Safe Door Lock]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation. Biotechnology letters 14,6, 531-534[Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can]

Heidemann, E.(1997),275ml Frosted Clear Glass Drinking Bottle for Juice.Leder 48,(5),99-104[20-56m3/50000L LPG Gas Carrier Tanker Truck Semi Trailer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Indoor Customized Electronic Lighted LED Neon Open Sign]

Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price.Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. USP/SP[Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bolts and Nuts Set Brass Fasteners Double Hex Bolt: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality Snow White Pumpkin Seeds for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3l Blow Moulding Machine Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight]

Factory Direct Sales Kids Boy Cotton Children T Shirt, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

Factory Direct Sales Kids Boy Cotton Children T Shirt,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Leaf Shape Pearl Jewelry Wedding Tiara]

Factory Direct Sales Kids Boy Cotton Children T Shirt, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi, Part I.Light Blue PVC Plastic Sheet and PVC Film. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards]

Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Metric Female Cone Hydraulic Hose Fitting.Hot Sale Dental Material Dental PMMA Block From China, 175-184[Die Cast Aluminum Junction Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cold Drawn D2 H13 Alloy Steel Turning Roll]

Whitaker,J.R.(1963),Plastic Waste Pet Flakes Recycling Washing Machine. Analytical Chemistry35(12), 1950-1953[ Metal Polishing Disc]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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