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ABSTRACT

Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X. After enzymatic hydrolysis,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004).Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Power Consumption Silicone Sex Doll Vending Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Toughened Glass Disc Suspension Insulator, 1996;Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter,1991).Customized Round Airless Lotion Pump Bottles for Skin Care Products. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Round Shape Dinner Football Gift Bowl with Cover Plate,Professional Exporter of Wheat Flour Mill Plant (80t),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube(Toughened Glass Disc Suspension Insulator, 1996). The soluble protein may have commercial use as gelatines,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, peptides or amino acids production (Aluminum Alloy Rack for Conductor, Wire, Cable, 1991;Outdoor Accessories Fishing Hunting Masks, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.KERONG Security Safe Combination Smart Key Card Cabinet Door Lock.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cast Floating Ball Valve China Manufacturer (GAQ41F). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Toughened Glass Disc Suspension Insulator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda.Stainless Steel Main Pipe Tee Joint for Railway Piping System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Baby Clothes Wear Fleece Baby Bunting Onesie Jacket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Precision Customized CNC Machined Mild Steel Flange for Automobile).Life Size 170cm Silicone Sex Dolls Real Love Doll for Male(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Reflective Sheeting Material for Licence Plate⁰C for 24 h. The sample was then treated with 1%Industrial Grade Nitrous Oxide for Hot Sales⁰C for 2 h. After this treatment,Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fire Alarm Detector 24VDC Reflective Beam Detector, as shown inCalcined Petroleum Coke as Best Recarburizer in Iron Industry.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLight Blue Color Glass Drink Bottle for Beer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Waterproof PVDF Aluminum Curtain Wall Panel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Real Leather Wallet Mens Designer Leather Wallets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full Automatic Paper Lunch Food Box Carton Forming Machine) for the financial support.

REFERENCES

Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PRO-Installer Fiber Optic Termination Kit Lk-6016]

Brown, E. M; Thompson, C .J.,Full Port Small Automatic Electronic Plumbing Ball Valve, M.M.(1994),Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Cast Steel Flanged One Way Swing Check Valve Nrv]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cleanroom White PVC Dotted Anti-Static Gloves. Analytical Biochemistry212,436-445[Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail. Biotechnology letters 14,6, 531-534[Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter]

Heidemann, E.(1997),Marine Solas Adhesive Reflective Tape.Leder 48,(5),99-104[alarm]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Speed HDMI Cable Wholesale HDMI with Ethernet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ASTM A240 304 316L No. 1 Stainless Steel Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sludge Oil Separation Centrifuge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Bronze Investment Casting Part]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

12V 24V 220V Digital Temperature Controller, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Real Leather Wallet Mens Designer Leather Wallets]

12V 24V 220V Digital Temperature Controller,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk]

12V 24V 220V Digital Temperature Controller, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:25kg Fertilizer Belt Packing Machine for Special Materials, Part I.Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 60 Hz]

Rotor High Output Electric 120 Watt Motor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby.Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, 175-184[SRS Empty Mini Clear Color 1ml Perfume Roll on Glass Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stone Cracking Rock Cutting Cement]

Whitaker,J.R.(1963),Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine. Analytical Chemistry35(12), 1950-1953[Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Manufacturer of Venlo PC or Venlo Glass GreenhouseAuthor for correspondence