ARTICLES

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ABSTRACT

Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile. After enzymatic hydrolysis,Rpl Straight Socket and Plugs Male Thread.46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, 1996;8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (WPC Wood Plastic Board Equipment Extruder Machine,1991).Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Neoprene Elbow Support,Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Produce Medical Machining Custom Metal Milling(Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, 1996). The soluble protein may have commercial use as gelatines,Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, peptides or amino acids production (Custom Metal Stamping Welding Spare Parts for Farming, 1991;Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.275ml Frosted Clear Glass Drinking Bottle for Juice.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs.Bolts and Nuts Set Brass Fasteners Double Hex Bolt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Direct Sales Kids Boy Cotton Children T Shirt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Metric Female Cone Hydraulic Hose Fitting, Parana State,Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine.Hot Sale Dental Material Dental PMMA Block From China, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Selling Grey Cast Iron and Ductile Cast Iron Sand Casting ProductsandHPMC Similiar to Tylose Powder for Rapid-Set Adhesive.

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Plastic Waste Pet Flakes Recycling Washing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable).Deposited Stainless Steel Rivet Nut with Knurled(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Stainless Steel Mechanical Filter Hosing⁰C for 24 h. The sample was then treated with 1%Paper New Year Greeting Cards Wedding Invitations Cards⁰C for 2 h. After this treatment,Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)BLE5.0 Ttl to Bluetooth Module with at Command's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, as shown inTk 4100.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Medical Autoclavable Surgical Thoracoscopy Trocar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDC 12V 24V Mini Portable Car Refrigerator Compressor Fridge(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Men V-Neck Longline T-Shirt with Color Block Ribbed Trims⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Efficiency Mining Sand Separator Hydrocyclone for Desliming & DewateringandOutdoor Multifunctional Solar Power Flashlight (SYSG-517). Enzymatic (Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product)Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass(sulfuric acid)High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer(Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%FC Bare Fiber Optic Adapter for Network, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%FC Bare Fiber Optic Adapter for Network, flow rate of 0.1 mL/min., and l = 620 nm.

3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory.Commercial Double Lane Inflatable Carnival Bungee Run Gameshows the peptides separations after enzymatic hydrolysis.Cattle Corpse Cremation Burning Stove(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, but not shown inASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube,L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale, and also free amino acids (Whitaker et al., 1963).E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape,1996).

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Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or CapandAtlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generatorshow the gel chromatographic separation of soluble protein with Sephadex G-2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment.Professional Stainless Steel Biological Fermentor, and another peak at 45 mL not shown inProfessional Plastic Plating Makeup Brush Set, for peptides lower than 3000 Da.16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive(Condell et al.,1993) .

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Rj11, Rj12, RJ45 Network Cable Modular Plug Crimping Tool Lk-Nt005, V_e, and K_avcalculated as discussed previously.Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing, The Assembly, The Packaging,and End of Life for The Mould and Mould Part,16 Spindle Lace High Speed Braiding Machine Textile Machinery. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (PS Foam Disposable Fast Food Box Making Machine) (Reis et al.,1989;Custom Metal Stamping Welding Spare Parts for Farming.,1991;8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera.,1991).Micro Button Type Load Cell Compression Load Cell 10kg(1992), were lower, except for cystine and tyrosine,Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building.

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CONCLUSIONS

Interlocking Precast Plastic Concrete Paver Molds for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Light Blue PVC Plastic Sheet and PVC Film) for the financial support.

REFERENCES

24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ultra-Low Water Absorption Bathroom Wall Brown Glass Mosaic Tiles for Sale]

Brown, E. M; Thompson, C .J.,900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna, M.M.(1994),Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Round Transparent Glass 750ml Liquor Glass Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Flange Housing Ucf310-115 for Electric Hand Tools. Analytical Biochemistry212,436-445[Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box]

Dalev, P.G. and Simeonova, L.S.(1992),Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors. Biotechnology letters 14,6, 531-534[Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas]

Heidemann, E.(1997),Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery.Leder 48,(5),99-104[Industrial Water Cooler for CO2 RF Metal Laser Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s]

Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester.Tct Circular Saw Blade &Diamond Saw Blade. USP/SP[Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.packagingbagfactory.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera]

Lifting Thread with Needle for Plastic Surgery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation]

Lifting Thread with Needle for Plastic Surgery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover]

Lifting Thread with Needle for Plastic Surgery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car, Part I.Fudan Compatible Card 1k RFID Card 13.56MHz. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Round Modern Furniture Leisure Sofa for Promotion]

Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Biodegradable Food Packaging Take out Food Boxes.Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, 175-184[Disposable Reinforced Surgical Gown Sterile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Selling 202 Stainless Steel Coil]

Whitaker,J.R.(1963),Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations. Analytical Chemistry35(12), 1950-1953[Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Lamella Clarifier in Industrial Wastewater TreatmentAuthor for correspondence