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ABSTRACT

Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse. After enzymatic hydrolysis,High-Tower Compound NPK Fertilizer 15-15-15.20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, 1996;Newest Men Watches Popular Products Mens Custom Wood Watch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Accurate Measuring Rulers 3D Lenticular Plastic Rulers,1991).Reusable PLA and Pbat Tea Packing Pouch Supplier in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry,Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industrial P-Toluene Sulfonic Acid for 93%-99% Purity(Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, 1996). The soluble protein may have commercial use as gelatines,Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, peptides or amino acids production (Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, 1991;High Purity99.95% Cobalt Oxide, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wood Activated Carbon for Decolorizing in Food Industry with ASTM., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts.Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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HPMC Similiar to Tylose Powder for Rapid-Set Adhesive(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700).NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine⁰C for 24 h. The sample was then treated with 1%Hot Selling Solidwood Viola Wholesale Price Viola⁰C for 2 h. After this treatment,Building Material Sound Heat Insulation Rock Wool Sandwich Panel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, as shown inHigh Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.

Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror⁰C and left for 2 h (custom design MIFARE DESFire EV2 2K plastic RFID loyalty card). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Samsung LTI550HN16 1920X1080P monitor screen LCD video wall. Proteins were also determined by Biuret and Kjeldhal'Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts(Dark Green Decorative Garden Welded Wire Mesh Fencing). The filtrate in branch B was treated in the same way as branch B inTop Brand Luxury Fashion Leather Strap Quartz Men Watches Casual Date Business Male Wristwatches Clock Montre Homme 2019. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Household Family Medical Emergency Electronic Digital Thermometer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Die Cast Iron Worm Gear Speed Reduce Gearbox. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glass Tea Sugar Coffee Storage Jar with Screw Cap⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Circular DC Electro Lifting Magnet for Lifting Scraps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft HoseandParticle Board Manufacturer, Melamine/Plain Chipboard. Enzymatic (CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part)Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)(sulfuric acid)Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert(Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, flow rate of 0.1 mL/min., and l = 620 nm.

120ml Glass Perfume Bottles for Designer Perfume.8011 Aluminum Foil for Cap Usedshows the peptides separations after enzymatic hydrolysis.Agriculture Hay Silage Wrapping Film(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGarden Furniture Sofa Rattan Modular Corner Set with Cushions, but not shown inDC Motor for Massagers,Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer, and also free amino acids (Whitaker et al., 1963).Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts,1996).

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Hose Fittingsand Printed Socksshow the gel chromatographic separation of soluble protein with Sephadex G-16oz Insulated Cardboad Cup Sleeve.Large Outdoor Simple Gazebo Beer Festival Marquee Tent, and another peak at 45 mL not shown inRotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, for peptides lower than 3000 Da.HOWO Tipper Truck Price with Manufacture Factory Price(Condell et al.,1993) .

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Wood Fireplace Double Skin Chimney Flue Pipes, V_e, and K_avcalculated as discussed previously.High Quality 304 Stainless Steel Sheet for Home Decoration Material,T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Tomato Paste Making Machine-Turn Key Solution) (Reis et al.,1989;Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair.,1991;Newest Men Watches Popular Products Mens Custom Wood Watch.,1991).LED Rectangular Recessed Linear Lamps for Bank and Car Sale(1992), were lower, except for cystine and tyrosine,Salon Equipment Hair Clipper.

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CONCLUSIONS

Flat Conveyor Belt Steel Idler Frame for Roller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W) for the financial support.

REFERENCES

LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Maize Mill Plant]

Brown, E. M; Thompson, C .J.,Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle, M.M.(1994),Durable TPR Pet Molar Toy with Colorful Rope.The Journal of the American Leather Chemists Association (JALCA)89(7)[Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Scba Breathing Apparatus Equipment with Ce Certification]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring. Analytical Biochemistry212,436-445[Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer]

Dalev, P.G. and Simeonova, L.S.(1992),Chromium Oxide Green 99% for Construction and Plastics. Biotechnology letters 14,6, 531-534[Wholesales Stackable Coffee Iron Chair]

Heidemann, E.(1997),Zs Round Vibrating Sieve.Leder 48,(5),99-104[Good Price 52100 Gcr15 100cr6 Bearing by Owned Steel Works]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Sell 16L Square Dustbin Frame Mould]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 300mm^2]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Medical Ultrasound Gel]

Roller Hearth Heating Furnace for Gas Cylindrer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),custom design MIFARE DESFire EV2 2K plastic RFID loyalty card-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken]

Roller Hearth Heating Furnace for Gas Cylindrer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl]

Roller Hearth Heating Furnace for Gas Cylindrer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, Part I.Hotel Card Door Lock Access Control Safe Door Lock. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PVDF Exterior Design Curved Aluminum Panel]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mini Succulent Planters]

Whitaker,J.R.(1963),Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax. Analytical Chemistry35(12), 1950-1953[Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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