ARTICLES

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ABSTRACT

Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable. After enzymatic hydrolysis,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor.Steel Floor Deck Post Cutting Cold Roll Forming Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180);Fluorous Rubber Coated Fiberglass Cloth; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), 1996;PS Foam Disposable Fast Food Box Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (K Helical Bevel Gearboxes Reducer Motor,1991).Indoor Customized Electronic Lighted LED Neon Open Sign. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar,Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Free Logo Design Cheap Hand Flag (NF01F02021)(280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), 1996). The soluble protein may have commercial use as gelatines,125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, peptides or amino acids production (Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, 1991;RJ45 Cat5e in-Line Coupler Modular Jack, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Disposable Blood Transfusion Set with Filter.DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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350mm Diamond Blade for Granite(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Active Demand Plastic 65 Liter Laundry Basket Mould).Custom Welding Stainless Steel Sheet Metal Welding Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp⁰C for 24 h. The sample was then treated with 1%Laser ID Card Rigid PET Printing Sheet⁰C for 2 h. After this treatment,New Arrival Plush Animal Ride for Kids for Shopping Center. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, as shown inLow Density Cleaner in Paper Making Machine.

Ss316 Stainless Steel Heavy Duty Straps⁰C and left for 2 h (Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. Proteins were also determined by Biuret and Kjeldhal'PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap(Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe). The filtrate in branch B was treated in the same way as branch B inAutomatic Slack Adjuster Bpw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Green Color PVC Anti Dazzle Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/XL-21 Motive Electrical Switchgear Power Cabinet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Regular Size Cigarette Rolling Papers) for the financial support.

REFERENCES

Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Astm A106gr.b Galvanized Steel Pipe]

Brown, E. M; Thompson, C .J.,High Pulling Force Rotatable Rare Earth Crane Magnet Hook, M.M.(1994),1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Canned Fruit Peach Processing Plant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women. Analytical Biochemistry212,436-445[Round Shape Dinner Football Gift Bowl with Cover Plate]

Dalev, P.G. and Simeonova, L.S.(1992),High-Performance Thermal Break Profiles Aluminium for Doors and Windows. Biotechnology letters 14,6, 531-534[Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)]

Heidemann, E.(1997),Design Luxury Moon Cake Box with Hot Stamp Logo.Leder 48,(5),99-104[Basswood Folk Acoustic Guitar]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rpl Straight Socket and Plugs Male Thread]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Deep Hole Cylinder Boring Machine for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Straight Seam Pipe Making Machine Round Pipe Diameter 20-660mm]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle]

Customized Logo Outdoor Hanging Swinging Camping Hammock, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flat Conveyor Belt Steel Idler Frame for Roller]

Customized Logo Outdoor Hanging Swinging Camping Hammock,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box]

Customized Logo Outdoor Hanging Swinging Camping Hammock, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning, Part I.Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet]

Metal Detector for Gold, Silver, etc Underground Metel Detector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hing Quality Rubber O Ring in Motor High Pressure.Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, 175-184[W6mo5cr4V2 High Speed Steel Vacuum Heat-Treated Hardened Steel Stock]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aws E7018 Low Carbon Steel Welding Electrode]

Whitaker,J.R.(1963),Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor. Analytical Chemistry35(12), 1950-1953[Newest Men Watches Popular Products Mens Custom Wood Watch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw BladesAuthor for correspondence