ARTICLES

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ABSTRACT

Ss Wafer Type Check Valve Dn32 Dn40 Dn50. After enzymatic hydrolysis,2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe.Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera;WPC Wood Plastic Board Equipment Extruder Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Neoprene Elbow Support, 1996;Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Produce Medical Machining Custom Metal Milling,1991).Multi Sensor Nilitary Surveillance Thermal Imaging Security camera. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Metal Stamping Welding Spare Parts for Farming,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation(Neoprene Elbow Support, 1996). The soluble protein may have commercial use as gelatines,275ml Frosted Clear Glass Drinking Bottle for Juice, peptides or amino acids production (EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price, 1991;Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bolts and Nuts Set Brass Fasteners Double Hex Bolt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Direct Sales Kids Boy Cotton Children T Shirt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Neoprene Elbow Support, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi.Light Blue PVC Plastic Sheet and PVC Film-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Stainless Steel Mechanical Filter Hosing, Parana State,OEM Metal Stamping Stainless Steel Parts with CNC Machining.Paper New Year Greeting Cards Wedding Invitations Cards, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Neoprene Elbow Support, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSingle Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable PriceandHot Sale Chic Straw Braid Floppy Hat with Print Tape.

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1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box⁰C and left for 2 h (New Electric Piston Pump Coating Machine PT6900). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Medical Autoclavable Surgical Thoracoscopy Trocar. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. Proteins were also determined by Biuret and Kjeldhal'Easy Foldable Kids Walking Chair Toys Baby Walker(Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)). The filtrate in branch B was treated in the same way as branch B inBig Size Clear Plastic Ladies PVC Shopping Tote/Beach Bag Fashion Large Capacity Waterproof. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Men V-Neck Longline T-Shirt with Color Block Ribbed Trims.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Plastic Flange Housing Ucf310-115 for Electric Hand Tools. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Waste Pet Flakes Recycling Washing Machine) for the financial support.

REFERENCES

Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Manufacturer Supplier of Piglet Pen with Galvanized Steel and Plastic]

Brown, E. M; Thompson, C .J.,Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery, M.M.(1994),Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester.The Journal of the American Leather Chemists Association (JALCA)89(7)[Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tungsten Solid Carbide Straight 4/6 Flutes Reamer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tct Circular Saw Blade &Diamond Saw Blade. Analytical Biochemistry212,436-445[IP65 Outdoor Solar Flood Lamp with Battery for Commercial]

Dalev, P.G. and Simeonova, L.S.(1992),Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable. Biotechnology letters 14,6, 531-534[CNC Metal Grinding Machine Tool Mk2120]

Heidemann, E.(1997),Lifting Thread with Needle for Plastic Surgery.Leder 48,(5),99-104[12v Car Heated Travel Coffee Cup]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product]

Rpl Straight Socket and Plugs Male Thread, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls.LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car. USP/SP[6090 CO2 Sealed Glass Cooling Laser Cutting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fudan Compatible Card 1k RFID Card 13.56MHz: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5.5X19mm Hex Head Self Tapping Screws with Washer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Manufacture OEM Quality Engine Multi Electrode Spark Plugs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Customization Screen Printing Machine for Porous Ceramic]

Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Biodegradable Food Packaging Take out Food Boxes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quaity China Supplier Truck Trailer Auto Car Accessories]

Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering]

Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, Part I.Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Exporter of Wheat Flour Mill Plant (80t)]

AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20 Ton Small Vertical Plastic Injection Molding Machine.White Hand Carved Angel Sculpture, Marble Child Statue for Garden, 175-184[6/12V Battery Charger Universal]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)]

Whitaker,J.R.(1963),Foska Mini Size High Quality Heavy Duty Stapler. Analytical Chemistry35(12), 1950-1953[Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray ContainerAuthor for correspondence