ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Factory Supply Straight Ball Joint Bearing 02b04. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wrinkle Effect Crease UV Drying Machine UV Curing System

ABSTRACT

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack. After enzymatic hydrolysis,Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector.20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CMP No Nc Metal Switch Black 25mm Illuminated Push Button, 1996;Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil,1991).Felt Disc Polishing Sanding Disc with Wool Felt Diam. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System,Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth(CMP No Nc Metal Switch Black 25mm Illuminated Push Button, 1996). The soluble protein may have commercial use as gelatines,240T goodeng water/oil/gas pipe drilling rig HDD machine, peptides or amino acids production (Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, 1991;Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Colorful Braided Fabric Cable Round.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Universal Wholesale Hot Sale Custom Blank Keys. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CMP No Nc Metal Switch Black 25mm Illuminated Push Button, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge.Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waw 8 Ton Logistics Light Truck for Transportation(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

BMC (DMC) / SMC Compression Moulding, Parana State,Wrinkle Effect Crease UV Drying Machine UV Curing System.Ring Caps &Plastic Tube Pop Novelties, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CMP No Nc Metal Switch Black 25mm Illuminated Push Button, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRal9002 White Prepainted Galvanized Steel Coil Z275 for Building Materialand600X600 mm Ugr 19 LED Panel Lights.

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Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Free Logo Design Cheap Hand Flag (NF01F02021)).Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dual Plastic 9 Volt Battery Clips Connector with Wire⁰C for 24 h. The sample was then treated with 1%Precision Ball Screw with Two Nut⁰C for 2 h. After this treatment,EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Low Density Cleaner in Paper Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada, as shown inCheap Wooden House Zinc Plated Angle Bracket.

Customized Stainless Steel Laser Cutting⁰C and left for 2 h (Microfiber Towel Car Drying Soft Towel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Price of a Retail Supermarket Push Cart for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable. Proteins were also determined by Biuret and Kjeldhal'OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate(Fused Silica Quartz Lump 5-50mm for Refractory Materials). The filtrate in branch B was treated in the same way as branch B inChina Acupressure Mat Ben Greenfield. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Handmade Natural Bamboo Basket / Bamboo Flower Pot.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Handrail Bar Holder for 16mm Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avShac Flange Linear Bearing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Router for Engraving Cutting Carving Drilling Milling, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, V_e, and K_avcalculated as discussed previously.China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal,CNC Machining Metal Mechanical Engineering Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags) (Reis et al.,1989;Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas.,1991;Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.,1991).Low Sulphur Calcined Carbon Raiser(1992), were lower, except for cystine and tyrosine,Hot Selling Free Sample Natural Fragrant Bath SPA Salt.

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CONCLUSIONS

Custom FSTN Va COB Cog Segment LCD Display Module. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply) for the financial support.

REFERENCES

Jc680 High Temperature Pressure Transmitter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cute Cat Face Kid Bag Children Bag Girl Crossbody Bag Coin Purse]

Brown, E. M; Thompson, C .J.,Deep Hole Cylinder Boring Machine for Sale, M.M.(1994),Hot Selling 202 Stainless Steel Coil.The Journal of the American Leather Chemists Association (JALCA)89(7)[2D LED Rope Lighting Motif Lights for Christmas Decoration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Colorful 100LED Copper String Light Outdoor LED]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-NPK 16X Hammer Spare Parts Racing Forged Piston. Analytical Biochemistry212,436-445[Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016]

Dalev, P.G. and Simeonova, L.S.(1992),2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve. Biotechnology letters 14,6, 531-534[Metal Cuting CNC Plasma Cutting Machine (DW1325)]

Heidemann, E.(1997),Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.Leder 48,(5),99-104[200W CCD Jewelry Laser Welder with Camera]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Mini Bag Spices Small Salt Packing Packaging Machine]

Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mobile Hydraulic Concrete Placing Boom.Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700. USP/SP[SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Deposited Stainless Steel Rivet Nut with Knurled]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Used Sport Inline Skate Shoes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Resistan UV Grades PP Non Woven Fabric]

Cr1000 Spray Nozzle Common Rail Injector Tester, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Cast Steel Flanged One Way Swing Check Valve Nrv-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester]

Cr1000 Spray Nozzle Common Rail Injector Tester,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)]

Cr1000 Spray Nozzle Common Rail Injector Tester, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Black White Stone 925 Silver Cross Pendants Necklace Jewelry, Part I.Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners]

Kt Board Acrylic CCD Camera CNC Knife Cutting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17.Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, 175-184[Granite Blade Cutting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mini Pencil Tag with Lanyard (YS211)]

Whitaker,J.R.(1963),Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. Analytical Chemistry35(12), 1950-1953[Waterproof PVDF Aluminum Curtain Wall Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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