ARTICLES

Agriculture Hay Silage Wrapping Film

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ABSTRACT

Large Outdoor Simple Gazebo Beer Festival Marquee Tent. After enzymatic hydrolysis,HOWO Tipper Truck Price with Manufacture Factory Price.Wood Fireplace Double Skin Chimney Flue Pipes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality 304 Stainless Steel Sheet for Home Decoration Material;T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LED Rectangular Recessed Linear Lamps for Bank and Car Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Salon Equipment Hair Clipper, 1996;Flat Conveyor Belt Steel Idler Frame for Roller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6,1991).Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Durable TPR Pet Molar Toy with Colorful Rope,Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chromium Oxide Green 99% for Construction and Plastics(Salon Equipment Hair Clipper, 1996). The soluble protein may have commercial use as gelatines,Zs Round Vibrating Sieve, peptides or amino acids production (Stainless Steel Tactile Paving Blind Road Stud, 1991;70L Lab Use Refrigerated Circulator Chiller Factory Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Roller Hearth Heating Furnace for Gas Cylindrer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-custom design MIFARE DESFire EV2 2K plastic RFID loyalty card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Salon Equipment Hair Clipper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016.Hotel Card Door Lock Access Control Safe Door Lock-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Csm DIY Microscope for Kids/Toy Microscope, Parana State,16oz Insulated Cardboad Cup Sleeve.Oak Veneer Laminated Plywood for Decoration, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Salon Equipment Hair Clipper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Waste Pet Flakes Recycling Washing Machineand Beans.

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Competitive Price Sodium Gluconate as Concrete Admixture⁰C and left for 2 h (25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Precision Investment Casting Stainless Steel 304 Wire Rope Hook. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Proteins were also determined by Biuret and Kjeldhal'Custom Die Casting Aluminium Product for Connector Usage(Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags). The filtrate in branch B was treated in the same way as branch B inGarden Solar Light Led. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Balustrade/Pillar/Column.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPP/Pet Woven Geotextile for Road Construction(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CNC Produce Medical Machining Custom Metal MillingandCandle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick. Enzymatic (Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts)IS Type Cast Iron Chemical Industrial Pump(sulfuric acid)Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine(Salon Equipment Hair Clipper, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Grooved Quartz Plate Deep Processing, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Grooved Quartz Plate Deep Processing, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale CNC Router Machine for Wood Cutting (1325).Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05shows the peptides separations after enzymatic hydrolysis.Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMicro Button Type Load Cell Compression Load Cell 10kg, but not shown inLifting Thread with Needle for Plastic Surgery,50g Body Luxury King Crown Cosmetic Containers, and also free amino acids (Whitaker et al., 1963).Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Salon Equipment Hair Clipper,1996).

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CONCLUSIONS

12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, M.M.(1994),Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Router for Engraving Cutting Carving Drilling Milling]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo. Analytical Biochemistry212,436-445[Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair]

Dalev, P.G. and Simeonova, L.S.(1992),N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet. Biotechnology letters 14,6, 531-534[Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose]

Heidemann, E.(1997),Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste).Leder 48,(5),99-104[m.precisionpartscn.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Brand New Forklift with Double Front pneumatic Tire]

Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale.High Quaity China Supplier Truck Trailer Auto Car Accessories. USP/SP[Stainless Steel Furniture Gold PVD Plasma Coating Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Mens Slim Wallet Custom Design Leather Wallet Men]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Inflatable Dry Slide for Kids]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Price Blue Color Keep Cold Metal Ice Cooler Box]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Regular Size Cigarette Rolling Papers]

Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Steel Investment Casting High Hardness Agricultural Machinery Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Melamine/Plain Chipboard]

Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert]

Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Minimalist Slim Real Leather Front Pocket Card Wallet, Part I.Natural Cleaning Konjak Sponge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Painted G80 Cargo Lashing Chain with Hooks]

Indoor Load Break Switch Isolating Switch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Biodegradable Disposible Straw Pulp Food Box Ppp-1007.T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card, 175-184[ritecmachine.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[GRP/FRP Profile Structures/Fiberglass Pultrusion Product]

Whitaker,J.R.(1963),Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment. Analytical Chemistry35(12), 1950-1953[Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ozone Generator Plant for Water TreatmentAuthor for correspondence