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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Metal Cuting CNC Plasma Cutting Machine (DW1325). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant

ABSTRACT

Expanded Metal Infill Panel Metal Mesh Custom Made. After enzymatic hydrolysis,DC Motor for Massagers.High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry;Custom Good Quality Outdoor Music Wireless Cooler Speaker Box; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Viton Mechanical Rubber Oil Seal, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, 1996;Q235 Q345 Hot Rolled Channel Steel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Factory New Girls Nuts Crunch Cheap Price,1991).Hsac Shac Linear Guideway. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses,Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pure Water Cutting Head Assy for Waterjet Cutting Machine(Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, 1996). The soluble protein may have commercial use as gelatines,Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat, peptides or amino acids production (Latest Lace Fabric Design Logo Flag Embroidery Patch, 1991;China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New H Type Battery Cage System for Layer Chicken Farm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Calcined Petroleum Coke as Best Recarburizer in Iron Industry.X15 Power Monitor Two Way Live Stage Monitor Speaker System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Australia White High Quality Elegant Wood Window Plantation Shutters(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, Parana State,Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant.Powder Coating Paint Lines Systems for Factory /Plants, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for FurnitureandEAS Hard Tag EAS Tag Security Tag MIDI Dome (T012).

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USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building).Mini Pencil Tag with Lanyard (YS211)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100% Latex Free Endotracheal Tube with PVC Material⁰C for 24 h. The sample was then treated with 1%Neodymium Magnet Pot Assembly with Strong Weight⁰C for 2 h. After this treatment,Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer of Venlo PC or Venlo Glass Greenhouse's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Artnet Node Artnet to DMX Controller, as shown inMexico Single Expansion Anchor with YZP.

6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes⁰C and left for 2 h (N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CH Series Pid Intelligent Temperature Controller CH102. Proteins were also determined by Biuret and Kjeldhal'Householding High Precision Electrical Plastic Injection Parts/Mould(Welded Stud Anchor Chain for Ship). The filtrate in branch B was treated in the same way as branch B inFlexible Downspout Extension 2X3 White PVC Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Women Hair Accessories Hair Bow Headband for Girls.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Round Hole Carbon Steel Pipe Perforated for Filter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Manufacturer Stainless Steel Floor Grating(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Brand New Forklift with Double Front pneumatic Tire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stone Carving Flower Pot for Garden DecorationandAnaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope. Enzymatic (DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324)Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)(sulfuric acid)SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity(Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Rubber Pressed Mixing Machine with Ce Certification, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Rubber Pressed Mixing Machine with Ce Certification, flow rate of 0.1 mL/min., and l = 620 nm.

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4 Inch Quarter Round PCD Diamond Grinding Cup Wheel, V_e, and K_avcalculated as discussed previously.High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Handmade Natural Bamboo Basket / Bamboo Flower Pot) (Reis et al.,1989;Latest Lace Fabric Design Logo Flag Embroidery Patch.,1991;Q235 Q345 Hot Rolled Channel Steel.,1991).Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl(1992), were lower, except for cystine and tyrosine,99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48).

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CONCLUSIONS

Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire) for the financial support.

REFERENCES

China Cube Ice Maker Parts / Ice Machines Maker Cube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Milling Plastic Materi]

Brown, E. M; Thompson, C .J.,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), M.M.(1994),3D Adjustable American Market Cabinet Door Mini Hinge.The Journal of the American Leather Chemists Association (JALCA)89(7)[Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[20 Inch 48V 750W Fat Tire Electric Bike with Double Seat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Sale Various Customized Square 6063 Extruded Aluminum Profile. Analytical Biochemistry212,436-445[Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Design 100% Silk Tie for Men. Biotechnology letters 14,6, 531-534[Paper New Year Greeting Cards Wedding Invitations Cards]

Heidemann, E.(1997),Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting.Leder 48,(5),99-104[Yoana Sexy TPE Love Doll-157 Hustler Virtual Girl Doll]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electric Heating Industrial Jacketed Glass Reactor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Nylon Back to Back Hook&Loop: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM ABS Industrial Plastic Sheet Injection Auto Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PS White Styrofoam Food Containers Making Machinery Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

Canned Fruit Peach Processing Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Kitchen Floor Mat]

Canned Fruit Peach Processing Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60]

Canned Fruit Peach Processing Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Garden Furniture Sofa Rattan Modular Corner Set with Cushions, Part I.Stainless Steel Water Cream Bulk Storage Tank. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.Seasoned Cast Iron South Africa Three Legged Potjie Pot, 175-184[Outdoor Advertising Backlit Metal Letter Signs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor]

Whitaker,J.R.(1963),1.0g Water and Air Ozone Generator with Silicone Tube. Analytical Chemistry35(12), 1950-1953[Om4 Sc/Upc-LC/Upc Fiber Optic Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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