ARTICLES

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^IICNPq/RHAE/biotechnology-Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Luxury Hotel Furniture Antique Design Marble Side Table

ABSTRACT

Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. After enzymatic hydrolysis,Commercial Double Lane Inflatable Carnival Bungee Run Game.Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, 1996;Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel,1991).High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100 Liter Cooking Pots for Jam,Food Grade Customized Metal Candy Can Square Mint Tin Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,600X600 mm Ugr 19 LED Panel Lights(5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, 1996). The soluble protein may have commercial use as gelatines,Good Quality Hot Tube8 Japanese Stainless Steel Tube 666, peptides or amino acids production (Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine, 1991;Round Plastic Hanging Basket (KD7014), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Design High Quality Alminum Sandwich Panel High Pressure Laminate.Steel Setractable Rail Protection Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Natural Birch F&B Veneer Furniture Plywood(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Spark Resistant Construction Axial Fan with Fixed Pitch Blade. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSilicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Precision CNC Milling DIN69871. Mas403 for CNC Machinist, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

360 Degree Maverick Angler Fishing Kayak with Rotating Seat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Performance 915MHz PCB Antenna) for the financial support.

REFERENCES

Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[British Type Hose Clamp]

Brown, E. M; Thompson, C .J.,Wholesale Flower Embroidery Applique Patches, M.M.(1994),Big Bussiness Project Commerical Chicken Poultry Farm Buildings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making. Analytical Biochemistry212,436-445[IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor]

Dalev, P.G. and Simeonova, L.S.(1992),Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. Biotechnology letters 14,6, 531-534[Om4 Sc/Upc-LC/Upc Fiber Optic Cable]

Heidemann, E.(1997),New Electric Piston Pump Coating Machine PT6900.Leder 48,(5),99-104[High Quality Solenoid Valve Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Wrap]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Crusher for Used Plastic Recycling Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Silicon Carbide Foundry Porous Ceramic Foam Filter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China 1 Ton Crawler Crane]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[X15 Power Monitor Two Way Live Stage Monitor Speaker System]

300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ceramic Bearing Si3n4 608-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Light Blue Color Glass Drink Bottle for Beer]

300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced]

300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nbm High Quality Auto Part Factory Price 6pk2310, Part I.Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Retail Shop Glass Display]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sc LC FC St Fiber Optical Adaptor Coupler Adapter]

Whitaker,J.R.(1963),Professional Manufacture Metal Medal Promotional Gift. Analytical Chemistry35(12), 1950-1953[Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)Author for correspondence