ARTICLES

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^IICNPq/RHAE/biotechnology-Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CMP No Nc Metal Switch Black 25mm Illuminated Push Button

ABSTRACT

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. After enzymatic hydrolysis,Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil.Felt Disc Polishing Sanding Disc with Wool Felt Diam. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System;Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (240T goodeng water/oil/gas pipe drilling rig HDD machine, 1996;Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength,1991).Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Colorful Braided Fabric Cable Round,Universal Wholesale Hot Sale Custom Blank Keys,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge(240T goodeng water/oil/gas pipe drilling rig HDD machine, 1996). The soluble protein may have commercial use as gelatines,Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, peptides or amino acids production (Waw 8 Ton Logistics Light Truck for Transportation, 1991;Hot Forging Lathe Spindle Forged Spindle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Chic Straw Braid Floppy Hat with Print Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lamella Clarifier in Sewage Tretament Plant for Water Purification. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (240T goodeng water/oil/gas pipe drilling rig HDD machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰BMC (DMC) / SMC Compression Moulding.Ring Caps &Plastic Tube Pop Novelties-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Low Density Cleaner in Paper Making Machine, Parana State,CMP No Nc Metal Switch Black 25mm Illuminated Push Button.En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (240T goodeng water/oil/gas pipe drilling rig HDD machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLED Neon Flex IP67 Waterproof Silicone DIY Neon LED StripandChromium Oxide Green 99% for Construction and Plastics.

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Customized Stainless Steel Laser Cutting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply).Price of a Retail Supermarket Push Cart for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable⁰C for 24 h. The sample was then treated with 1%OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate⁰C for 2 h. After this treatment,Handmade Natural Bamboo Basket / Bamboo Flower Pot. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Handrail Bar Holder for 16mm Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Shac Flange Linear Bearing, as shown inOEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate.

China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price⁰C and left for 2 h (Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Router for Engraving Cutting Carving Drilling Milling. Proteins were also determined by Biuret and Kjeldhal'Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine(Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo). The filtrate in branch B was treated in the same way as branch B inModern Design Hotel Project LED Floor Lamps. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Hot Rolled Steel U Channel China Supplier(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human HairandYCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable. Enzymatic (Anti-Pollution Modified Acrylic Solid Surface for Countertops)Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose(sulfuric acid)Corrugated Carton Box Fully Automatic Folding Gluing Machine(240T goodeng water/oil/gas pipe drilling rig HDD machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%800lb 1/2 A105 Thread Forged Steel Globe Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%800lb 1/2 A105 Thread Forged Steel Globe Valve, flow rate of 0.1 mL/min., and l = 620 nm.

Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence. etc Underground Metel Detectorshows the peptides separations after enzymatic hydrolysis.China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLatest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove, but not shown inLaser Cut Sheet Metal Fiber Laser Cutting Machine,CNC Machining Metal Mechanical Engineering Parts, and also free amino acids (Whitaker et al., 1963).Low Sulphur Calcined Carbon Raiser. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (240T goodeng water/oil/gas pipe drilling rig HDD machine,1996).

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Double Shaft Paper Cardboard Waste Plastic Shredder MachineandBuilding Material of Granite Ceramic Wall Tile for Wall Decorationshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Selling Free Sample Natural Fragrant Bath SPA Salt.Custom FSTN Va COB Cog Segment LCD Display Module, and another peak at 45 mL not shown inPlastic Flange Housing Ucf310-115 for Electric Hand Tools, for peptides lower than 3000 Da.Jc680 High Temperature Pressure Transmitter(Condell et al.,1993) .

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Deep Hole Cylinder Boring Machine for Sale, V_e, and K_avcalculated as discussed previously.Hot Selling 202 Stainless Steel Coil,NPK 16X Hammer Spare Parts Racing Forged Piston. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Purity Sodium Bicarbonate with White Powder Type) (Reis et al.,1989;Waw 8 Ton Logistics Light Truck for Transportation.,1991;Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas.,1991).2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve(1992), were lower, except for cystine and tyrosine,Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.

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CONCLUSIONS

Mobile Hydraulic Concrete Placing Boom. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision Ball Screw with Two Nut) for the financial support.

REFERENCES

Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Solar Powered Rechargeable Stand Fan]

Brown, E. M; Thompson, C .J.,Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, M.M.(1994),Cr1000 Spray Nozzle Common Rail Injector Tester.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lamella Clarifier in Sewage Tretament Plant for Water Purification]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Interlocking Precast Plastic Concrete Paver Molds for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. Analytical Biochemistry212,436-445[Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder]

Dalev, P.G. and Simeonova, L.S.(1992),Black White Stone 925 Silver Cross Pendants Necklace Jewelry. Biotechnology letters 14,6, 531-534[Gasoline Rototiller]

Heidemann, E.(1997),Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People.Leder 48,(5),99-104[Bench Button Rock Drill Bits]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture]

Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Kt Board Acrylic CCD Camera CNC Knife Cutting Machine.High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17. USP/SP[Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Steel Wire Reinforced Pipe Hose for Transfer Water Oil and Powder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale]

Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building]

Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D Adjustable American Market Cabinet Door Mini Hinge]

Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part, Part I.Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Wholesale Melamine Garment Shop Furniture Design for Store]

Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator.Carbon Steel Stainless Steel Countersunk Head Hollow Bolt, 175-184[Plastic Mould Factory/Plastic Mould for Sale/Plastic Mould Injection]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ltmg Classic Product 6 Ton Wheel Front Loader for Sale]

Whitaker,J.R.(1963),Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses. Analytical Chemistry35(12), 1950-1953[China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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