ARTICLES

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ABSTRACT

High Quality Flange Dismantling Joint. After enzymatic hydrolysis,Duplex Stainless Steel Floating Ball Valve.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine;Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, 1996;7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence,1991).Kubota Combine Harvester Spare Parts 5h484-2392 Nut. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Steel Floor Deck Post Cutting Cold Roll Forming Machine(CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, 1996). The soluble protein may have commercial use as gelatines,Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), peptides or amino acids production (Fluorous Rubber Coated Fiberglass Cloth, 1991;Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PS Foam Disposable Fast Food Box Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-K Helical Bevel Gearboxes Reducer Motor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Indoor Customized Electronic Lighted LED Neon Open Sign.Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Wire).High Quality Disposable Blood Transfusion Set with Filter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle⁰C for 24 h. The sample was then treated with 1%Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)⁰C for 2 h. After this treatment,High Quality Brushless DC Electric Motor for Industrial Deduster. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Factory Light Soda Ash /Sodium Carbonate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Regular Size Cigarette Rolling Papers, as shown inPrecision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.

304L Stainless Steel U Channel Good Performance⁰C and left for 2 h (Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Junior Backyard Training Practise Soccer Goal Net. Proteins were also determined by Biuret and Kjeldhal'350mm Diamond Blade for Granite(Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector). The filtrate in branch B was treated in the same way as branch B inFancy Fashion Girls Tulle Dress for Kids Clothes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Welding Stainless Steel Sheet Metal Welding Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLaser ID Card Rigid PET Printing Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ss316 Stainless Steel Heavy Duty Straps, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine.China Male Male Sanitary Steel Butterfly Valve with Square Pull Handleshows the peptides separations after enzymatic hydrolysis.New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, but not shown inCeramic Bearing Si3n4 608,Green Color PVC Anti Dazzle Panel, and also free amino acids (Whitaker et al., 1963).ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Machine Aluminum Telescopic Covers Flat Bellows Cover,1996).

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CONCLUSIONS

Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X) for the financial support.

REFERENCES

Hot Sale 840 Type PPGI Color Steel Roofing Sheets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Free Samples Strong Adhesive Custom Logo Printed BOPP Packing Tape]

Brown, E. M; Thompson, C .J.,FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, M.M.(1994),CATV Indoor Optical Receiver FTTH.The Journal of the American Leather Chemists Association (JALCA)89(7)[NBR Latex Adhesive for Glove]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Sell 16L Square Dustbin Frame Mould. Analytical Biochemistry212,436-445[Rht-060ws Soda Process Water Cooled Screw Type Water Chiller]

Dalev, P.G. and Simeonova, L.S.(1992),Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover. Biotechnology letters 14,6, 531-534[New Electric Piston Pump Coating Machine PT6900]

Heidemann, E.(1997),2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush.Leder 48,(5),99-104[Supermarkets and Stores Advertising X Banner Display]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)]

Foldable PP Plastic Storage Logistics Turnover Box, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml.PVDF Exterior Design Curved Aluminum Panel. USP/SP[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1200mm Recessed Mounted LED Linear Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Neodymium Magnet with RoHS ISO 16499 Approved: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lifting Eqipment Wire Rope Winch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Crusher for Used Plastic Recycling Line]

Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Accessories Fishing Hunting Masks]

Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine]

Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Pulling Force Rotatable Rare Earth Crane Magnet Hook, Part I.1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Low Sulphur Calcined Carbon Raiser]

Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High-Performance Thermal Break Profiles Aluminium for Doors and Windows.Design Luxury Moon Cake Box with Hot Stamp Logo, 175-184[T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[12V 24V 220V Digital Temperature Controller]

Whitaker,J.R.(1963),Standard Industrial Double Nut Ball Screw Dfu Series. Analytical Chemistry35(12), 1950-1953[(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cattle Corpse Cremation Burning StoveAuthor for correspondence