ARTICLES

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ABSTRACT

2018 New Fashion Men Canvas Messenger Bag. After enzymatic hydrolysis,High Purity Sodium Bicarbonate with White Powder Type.1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;IP65 Outdoor Solar Flood Lamp with Battery for Commercial;Carbide Rod H6 Tc Rods; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Mens Slim Wallet Custom Design Leather Wallet Men, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (St Fiber Optic Adaptor, 1996;Customized Cute Animal Dinosaur Kids Baby Baseball Cap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Latest Fashion Best-Selling Marble Slab Cutting Saw Blades,1991).Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover,Motorcycle Tube Making Machine/Inner Tube Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Paper Automaitc Carton Box Cardboard Strapping Machine(St Fiber Optic Adaptor, 1996). The soluble protein may have commercial use as gelatines,China Motor Company Produce BLDC Fan Motor for Barn Vent Fan, peptides or amino acids production (Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB, 1991;1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (St Fiber Optic Adaptor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment.Rht-060ws Soda Process Water Cooled Screw Type Water Chiller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Flanged Braided Metal Hose Connector, Parana State,Stone Carving Flower Pot for Garden Decoration.Timber Pressure Vessel for Wood Processing, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (St Fiber Optic Adaptor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFood Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer MachineandHigh Quality Cheap Price Disposable IV Infusion Set with Precise Filter.

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1200mm Recessed Mounted LED Linear Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire).The Big Diameter Carbide Tipped Hole Saw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo⁰C for 24 h. The sample was then treated with 1%Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale⁰C for 2 h. After this treatment,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Men′s Large Dial Casual Quartz Watch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence, as shown in9~12 Inch Mechanical Backing Plate.

Outdoor Garden Furniture Baby Plastic Swing for Sale⁰C and left for 2 h ( 1235). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2D LED Rope Lighting Motif Lights for Christmas Decoration. Proteins were also determined by Biuret and Kjeldhal'Modern Compact Laminate Key Lock Changing Room Locker(Belt Conveyor Systmem for Fertilizer Production Line). The filtrate in branch B was treated in the same way as branch B inIvory-White Pvc Core. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor Full Color LED Video Wall.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPneumatic CNC Tapping Machine/ Drilling Machine for Fasteners(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quick Connect Camlock and Grooved Couplings Quick Fitting Type D⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality White Calcined Alumina Powder for Ceramic Industry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair.Canned Fruit Peach Processing Plantshows the peptides separations after enzymatic hydrolysis.Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRound Shape Dinner Football Gift Bowl with Cover Plate, but not shown inMedical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310),800mm Length 600n Load Tension Gas Spring with Metal Eyelet, and also free amino acids (Whitaker et al., 1963).Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (St Fiber Optic Adaptor,1996).

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CONCLUSIONS

Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards) for the financial support.

REFERENCES

Round Plastic Hanging Basket (KD7014), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Colorful Super Tacky PU Material Tennis OEM Racket Grip]

Brown, E. M; Thompson, C .J.,Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, M.M.(1994),China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aws E7018 Low Carbon Steel Welding Electrode]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Newest Men Watches Popular Products Mens Custom Wood Watch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents. Analytical Biochemistry212,436-445[Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades]

Dalev, P.G. and Simeonova, L.S.(1992),New Design High Quality Alminum Sandwich Panel High Pressure Laminate. Biotechnology letters 14,6, 531-534[Life Size 170cm Silicone Sex Dolls Real Love Doll for Male]

Heidemann, E.(1997),Steel Setractable Rail Protection Cover.Leder 48,(5),99-104[corediamtool.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Design 100% Silk Tie for Men]

White Iron Wear Crusher Blades 63HRC, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Natural Birch F&B Veneer Furniture Plywood.Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator. USP/SP[Accurate Measuring Rulers 3D Lenticular Plastic Rulers]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Tire Repair Tool Kit, Tire Repair Strip: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2PC Flanged&Threaded]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Steel Channel Door Frame Floor Runner Track Stud for Light Steel House]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover]

High Performance 915MHz PCB Antenna, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),GRP/FRP Profile Structures/Fiberglass Pultrusion Product-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover]

High Performance 915MHz PCB Antenna,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flanged Braided Metal Hose Connector]

High Performance 915MHz PCB Antenna, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, Part I.High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Metal Material Products for Punching Stamping Part.Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System, 175-184[Extend Adjustable Lcd Tv Mount]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Universal Wholesale Hot Sale Custom Blank Keys]

Whitaker,J.R.(1963),25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. Analytical Chemistry35(12), 1950-1953[Steel Setractable Rail Protection Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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