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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Neodymium Magnet Pot Assembly with Strong Weight. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System

ABSTRACT

China Manufacturer of Venlo PC or Venlo Glass Greenhouse. After enzymatic hydrolysis,Artnet Node Artnet to DMX Controller.6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts;CH Series Pid Intelligent Temperature Controller CH102; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Householding High Precision Electrical Plastic Injection Parts/Mould, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Women Hair Accessories Hair Bow Headband for Girls, 1996;Round Hole Carbon Steel Pipe Perforated for Filter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Manufacturer Stainless Steel Floor Grating,1991).Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose,Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Brand New Forklift with Double Front pneumatic Tire(Fashion Women Hair Accessories Hair Bow Headband for Girls, 1996). The soluble protein may have commercial use as gelatines,Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope, peptides or amino acids production (DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, 1991;Ce Quality Double Channel Medical Syringe Pump (THR-SP180D), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Rubber Pressed Mixing Machine with Ce Certification.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Women Hair Accessories Hair Bow Headband for Girls, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4K Giant Whiteboard Interactive Panel for University Whiteboard.Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, Parana State,Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System.Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fashion Women Hair Accessories Hair Bow Headband for Girls, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016andMetal Cuting CNC Plasma Cutting Machine (DW1325).

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Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel).99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump⁰C for 24 h. The sample was then treated with 1%China Cube Ice Maker Parts / Ice Machines Maker Cube⁰C for 2 h. After this treatment,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3D Adjustable American Market Cabinet Door Mini Hinge's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, as shown inPolyester Hunting Clothes Breathable Sniper Army Airsoft Uniform.

Custom Design 100% Silk Tie for Men⁰C and left for 2 h (Deposited Stainless Steel Rivet Nut with Knurled). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover. Proteins were also determined by Biuret and Kjeldhal'Om4 Sc/Upc-LC/Upc Fiber Optic Cable(Resistan UV Grades PP Non Woven Fabric). The filtrate in branch B was treated in the same way as branch B inHot Latest Sexy Womens Gym Wear Online. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Nylon Back to Back Hook&Loop.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Canned Fruit Peach Processing Plant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQuartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Water Cream Bulk Storage Tank⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Round Transparent Glass 750ml Liquor Glass Bottle, V_e, and K_avcalculated as discussed previously.3D Creative Design Metal Medal Promotion Gift of Sport,Ad-L13 Black Perfume Plastics Vial Bottle 10ml. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price) (Reis et al.,1989;DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324.,1991;Round Hole Carbon Steel Pipe Perforated for Filter.,1991).Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover(1992), were lower, except for cystine and tyrosine,EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012).

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CONCLUSIONS

60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Practical Edible Silicone Kitchenware (YB-ty-29)) for the financial support.

REFERENCES

Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Esd Safety Shoes Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, M.M.(1994),Mexico Single Expansion Anchor with YZP.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fluorous Rubber Coated Fiberglass Cloth]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. Analytical Biochemistry212,436-445[Building Wall Machine for Hot Machine]

Dalev, P.G. and Simeonova, L.S.(1992),SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. Biotechnology letters 14,6, 531-534[Best Price Graphite Electrode HP 300mm UHP]

Heidemann, E.(1997),Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope.Leder 48,(5),99-104[Non Stick Heat Resistant Baking Paper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-200m Diesel Oil Hydraulic Water Well Drilling Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Polypropylene Strapping Band Production Line/PP Strap Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[19 Inch Rack Standard]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Custom Cmyk Printing Cake Gift Packing Box with Lid, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Air Purification Activated Alumina-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Furniture Grade E1 Glue Plywood with Competitive Price]

Custom Cmyk Printing Cake Gift Packing Box with Lid,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine]

Custom Cmyk Printing Cake Gift Packing Box with Lid, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6), Part I.Resistan UV Grades PP Non Woven Fabric. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box]

Whitaker,J.R.(1963),Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. Analytical Chemistry35(12), 1950-1953[Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chemical HPMC Kraft Paper Bag with PP Woven Fabric LaminatedAuthor for correspondence