ARTICLES

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ABSTRACT

N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet. After enzymatic hydrolysis,Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste).Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, 1996;Minimalist Slim Real Leather Front Pocket Card Wallet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Natural Cleaning Konjak Sponge,1991).Indoor Load Break Switch Isolating Switch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Biodegradable Disposible Straw Pulp Food Box Ppp-1007,T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment(Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, 1996). The soluble protein may have commercial use as gelatines,NBR Latex Adhesive for Glove, peptides or amino acids production (Cheap Wooden House Zinc Plated Angle Bracket, 1991;4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer.500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12 Inch King Mattress Latex Foam Memory Foam Mattress(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Longyi Crimping Types Cable Terminal Lugs, Parana State,Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo.Rigid PVC Foam Board for FRP Marine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePractical Edible Silicone Kitchenware (YB-ty-29)andShac Flange Linear Bearing.

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Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge).41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Furniture Gold PVD Plasma Coating Machine⁰C for 24 h. The sample was then treated with 1%Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment⁰C for 2 h. After this treatment,Steel Pipe Rust Removal Shot Blasting Cleaning Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hydraulic Press Corrugated Cold Roll Automatically Forming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, as shown inUnbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp.

Automatic Bread Crumb Processing Line and Making Extruder Machine⁰C and left for 2 h (100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring. Proteins were also determined by Biuret and Kjeldhal'300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve(Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding). The filtrate in branch B was treated in the same way as branch B inWholesale 5PCS/Set Multi Color Rubber Golf Tees 83mm. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Injection Molding Companies China for Tablet PC Case.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Solid Tungsten Carbide Rods(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Tensisle Strength Tissue Paper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tractor Mounted Beach Cleaning Equipment Large Size, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mining Machinery Parts Diamond Saw Blade Segments for Marble.Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3shows the peptides separations after enzymatic hydrolysis.Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlange Type Ball Float Water Tank Control Valve, but not shown inMen V-Neck Longline T-Shirt with Color Block Ribbed Trims,Multi Column Load Cell 100kg, 20t, and also free amino acids (Whitaker et al., 1963).LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Steel Investment Casting High Hardness Agricultural Machinery Parts,1996).

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Buddhist Mood Picture Wallpaper Murals, V_e, and K_avcalculated as discussed previously.High Quality Flange Dismantling Joint,Duplex Stainless Steel Floating Ball Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Professional Manufacturer Stainless Steel Floor Grating) (Reis et al.,1989;Cheap Wooden House Zinc Plated Angle Bracket.,1991;Minimalist Slim Real Leather Front Pocket Card Wallet.,1991).E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card(1992), were lower, except for cystine and tyrosine,Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine.

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CONCLUSIONS

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt) for the financial support.

REFERENCES

Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Jinma Tractor Xinchai Diesel Engine Parts C490bpg Injection Pump]

Brown, E. M; Thompson, C .J.,CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, M.M.(1994),7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PP/Pet Woven Geotextile for Road Construction]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence. Analytical Biochemistry212,436-445[Plastic Injection Molding Companies China for Tablet PC Case]

Dalev, P.G. and Simeonova, L.S.(1992),Kubota Combine Harvester Spare Parts 5h484-2392 Nut. Biotechnology letters 14,6, 531-534[Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Cmyk Printing Cake Gift Packing Box with Lid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hing Quality Rubber O Ring in Motor High Pressure]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hand Hammered Carbon Steel Wok with Single Wooden Handle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hotel Card Door Lock Access Control Safe Door Lock]

Fluorous Rubber Coated Fiberglass Cloth, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can]

Fluorous Rubber Coated Fiberglass Cloth,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Indoor Customized Electronic Lighted LED Neon Open Sign]

Fluorous Rubber Coated Fiberglass Cloth, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), Part I.PS Foam Disposable Fast Food Box Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine]

K Helical Bevel Gearboxes Reducer Motor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Indoor Customized Electronic Lighted LED Neon Open Sign.Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, 175-184[7 In Stock - Beta Arbutin Powder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

Whitaker,J.R.(1963),Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. Analytical Chemistry35(12), 1950-1953[Good Quality Snow White Pumpkin Seeds for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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