ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Femo Ferro Molybdenum Powder Lump 55/60. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight

ABSTRACT

Round Panoramic Lift, Glass Observation Passenger Elevator. After enzymatic hydrolysis,Painting Road Marking Vehicles Machine for Sale.Automotive Rubber Parts for Any Rubber Material. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CREE Chip 24W 28W 30W LED Downlight, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, 1996;Three Phase 35kv Current Transformer for Indoor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Shipping Logistics to Stockholm,1991).CNC Lathe Main Parts with Good Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Price High Torque 12mm Low Speed Small Geared Motor,Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man(Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, 1996). The soluble protein may have commercial use as gelatines,Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting, peptides or amino acids production (Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated, 1991;Mashroom Head Nylon Anchor with Pozi Nail, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterjet 87K Intensifier Part Check Valve Body 020077-1.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Meat Machinery/Chicken Breast and Fish Slicing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine.1.0mm Binding Wire Galvanized Iron Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Microfiber Towel Car Drying Soft Towel, Parana State,8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight.Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfessional Various Standard Hot Sale Rotary Lab Single Round PunchandPoultry Farming Equipment Broiler Chicken Ground Floor Rearing System.

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High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (St Fiber Optic Adaptor).STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine⁰C for 24 h. The sample was then treated with 1%New Styles Fashion Erotic Women Underwear Sexy Lingerie⁰C for 2 h. After this treatment,High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm, as shown inT5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card.

Fully Automatic Water Bottle Lids Labeling Machine⁰C and left for 2 h (High Quality Melamine Paper Manufacturer for Decoration). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera. Proteins were also determined by Biuret and Kjeldhal'Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs(925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring). The filtrate in branch B was treated in the same way as branch B inchildren outdoor playhouse. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fused Silica Quartz Lump 5-50mm for Refractory Materials.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cold Drawn D2 H13 Alloy Steel Turning Roll. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInterlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Colorful 100LED Copper String Light Outdoor LED, pH 3.25., mobile phase B: buffer solution of 0.01 mol/H365 Emas Chainsaw Spares Cylinder Kits, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED LightandPP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg. Enzymatic (A4 Diploma Covers Wholesale with Silver Foil)OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings(sulfuric acid)Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap(Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%120L 240L Oil Universal Chemical Spil Absorbent Kits, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%120L 240L Oil Universal Chemical Spil Absorbent Kits, flow rate of 0.1 mL/min., and l = 620 nm.

Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse.Digger Spring for Excavator Machineryshows the peptides separations after enzymatic hydrolysis.High-Tower Compound NPK Fertilizer 15-15-15(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSimilar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), but not shown inCNC Machining Metal Mechanical Engineering Parts,20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, and also free amino acids (Whitaker et al., 1963).8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove,1996).

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Newest Men Watches Popular Products Mens Custom Wood Watch, V_e, and K_avcalculated as discussed previously.Accurate Measuring Rulers 3D Lenticular Plastic Rulers,Reusable PLA and Pbat Tea Packing Pouch Supplier in China. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Precision Mold Manufacturer Plastic Injection Mould) (Reis et al.,1989;Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated.,1991;Three Phase 35kv Current Transformer for Indoor.,1991).Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry(1992), were lower, except for cystine and tyrosine,Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold.

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CONCLUSIONS

Industrial P-Toluene Sulfonic Acid for 93%-99% Purity. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder) for the financial support.

REFERENCES

Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Car Molding for OEM Molded Injection Plastic Parts]

Brown, E. M; Thompson, C .J.,Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, M.M.(1994),High Purity99.95% Cobalt Oxide.The Journal of the American Leather Chemists Association (JALCA)89(7)[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wood Activated Carbon for Decolorizing in Food Industry with ASTM. Analytical Biochemistry212,436-445[ Tire Repair Strip]

Dalev, P.G. and Simeonova, L.S.(1992),Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car. Biotechnology letters 14,6, 531-534[400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making]

Heidemann, E.(1997),Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine.Leder 48,(5),99-104[Akmj Series Capacitor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Acid Water Pulse Output Liquid Orifice Type Flow Meter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Meat Machinery/Chicken Breast and Fish Slicing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Window System Thermal Break Strip (HK Stype35.3mm)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Din934 Nut]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X]

6000 Series Anodizing Extrusion Aluminum Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Die Casting Zinc Alloy Badge Use for Car]

6000 Series Anodizing Extrusion Aluminum Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ampoule Sealer Machine Hydrogen Oxygen Gas Generator]

6000 Series Anodizing Extrusion Aluminum Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, Part I.Tomato Paste Making Machine-Turn Key Solution. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk]

Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine.HPMC Similiar to Tylose Powder for Rapid-Set Adhesive, 175-184[www.dmmccb.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IS Type Cast Iron Chemical Industrial Pump]

Whitaker,J.R.(1963),NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple. Analytical Chemistry35(12), 1950-1953[Starter Motor 0001367034 12V 3KW 9T for Mercedes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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