ARTICLES

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ABSTRACT

HOWO Tipper Truck Price with Manufacture Factory Price. After enzymatic hydrolysis,Wood Fireplace Double Skin Chimney Flue Pipes.High Quality 304 Stainless Steel Sheet for Home Decoration Material. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant;LED Rectangular Recessed Linear Lamps for Bank and Car Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Salon Equipment Hair Clipper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Flat Conveyor Belt Steel Idler Frame for Roller, 1996;LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle,1991).Durable TPR Pet Molar Toy with Colorful Rope. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring,Chromium Oxide Green 99% for Construction and Plastics,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Zs Round Vibrating Sieve(Flat Conveyor Belt Steel Idler Frame for Roller, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Tactile Paving Blind Road Stud, peptides or amino acids production (70L Lab Use Refrigerated Circulator Chiller Factory Price, 1991;Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Roller Hearth Heating Furnace for Gas Cylindrer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.custom design MIFARE DESFire EV2 2K plastic RFID loyalty card.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Flat Conveyor Belt Steel Idler Frame for Roller, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hotel Card Door Lock Access Control Safe Door Lock.Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel DIN7981 Pan Head Self Tapping Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Oak Veneer Laminated Plywood for Decoration, Parana State,Large Outdoor Simple Gazebo Beer Festival Marquee Tent.China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Flat Conveyor Belt Steel Idler Frame for Roller, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theVegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull ChopperandUSB LED Clock Fan USB-Powered Portable Fan with Clock.

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Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment).High Speed HDMI Cable Wholesale HDMI with Ethernet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine⁰C for 24 h. The sample was then treated with 1%High Quality Women′s Suit in Reasonable Price⁰C for 2 h. After this treatment,Pet Short Fiber Non Woven Geotextile for Landfill Filtration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Competitive Price Sodium Gluconate as Concrete Admixture, as shown in13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System.

Precision Investment Casting Stainless Steel 304 Wire Rope Hook⁰C and left for 2 h (Longyi Crimping Types Cable Terminal Lugs). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Die Casting Aluminium Product for Connector Usage. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Balustrade/Pillar/Column(Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion). The filtrate in branch B was treated in the same way as branch B inAviation Paper Cup Counting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PP/Pet Woven Geotextile for Road Construction. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLamella Clarifier in Industrial Wastewater Treatment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CNC Metal Grinding Machine Tool Mk2120, V_e, and K_avcalculated as discussed previously.Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket,Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Noiseless) Cracking Agent (SCA) for Breaking Rocks) (Reis et al.,1989;70L Lab Use Refrigerated Circulator Chiller Factory Price.,1991;LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6.,1991).Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM(1992), were lower, except for cystine and tyrosine,12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch.

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CONCLUSIONS

Hydraulic Electric Stationary Car Platform Scissor Elevator Lift. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dark Green Decorative Garden Welded Wire Mesh Fencing) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal, M.M.(1994),Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Compost Turner Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet. Analytical Biochemistry212,436-445[Latest Fashion Best-Selling Marble Slab Cutting Saw Blades]

Dalev, P.G. and Simeonova, L.S.(1992),Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste). Biotechnology letters 14,6, 531-534[Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller]

Heidemann, E.(1997),Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale.Leder 48,(5),99-104[Excavator Pre Air Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Iron Wire Mesh Chain Link Mesh Security Fence]

Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quaity China Supplier Truck Trailer Auto Car Accessories.Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable. USP/SP[High Quality Brushless DC Electric Motor for Industrial Deduster]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sicher Elevator Hospital Bed Elevator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Roller Hearth Heating Furnace for Gas Cylindrer]

Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Minimalist Slim Real Leather Front Pocket Card Wallet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CMP No Nc Metal Switch Black 25mm Illuminated Push Button]

Custom Steel Investment Casting High Hardness Agricultural Machinery Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Pulling Force Rotatable Rare Earth Crane Magnet Hook]

Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Natural Cleaning Konjak Sponge, Part I.Indoor Load Break Switch Isolating Switch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar]

Biodegradable Disposible Straw Pulp Food Box Ppp-1007, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card.Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment, 175-184[Orbital Steering / Hydraulic Steering Control Unit Thoth/Zihyd Hydraulic Motors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car]

Whitaker,J.R.(1963),NBR Latex Adhesive for Glove. Analytical Chemistry35(12), 1950-1953[Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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