ARTICLES

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ABSTRACT

Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator. After enzymatic hydrolysis,High Quality Tire Repair Tool Kit, Tire Repair Strip.High Performance 915MHz PCB Antenna. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, 1996;Customized Metal Material Products for Punching Stamping Part., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System,1991).25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L,Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Theme Park Inflatable Bouncer for Sale (BJ-B16)(Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, 1996). The soluble protein may have commercial use as gelatines,Muliti-Size Light Hot Stamping Foil for PS Frame, peptides or amino acids production (Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, 1991;50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China 1 & 100 RMB Acrylic Currence Frame., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1 Ton Toyo Type Manual Chain Block.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Spark Resistant Construction Axial Fan with Fixed Pitch Blade. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl.3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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White Desiccant Bag Silica Gel with Indicated Orange Bead⁰C and left for 2 h (Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Iron Wire Mesh Chain Link Mesh Security Fence. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump. Proteins were also determined by Biuret and Kjeldhal'5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide(Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers). The filtrate in branch B was treated in the same way as branch B inNew Product Ideas 2019 Wholesale Reusable Silicone Food Storage Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av360 Degree Maverick Angler Fishing Kayak with Rotating Seat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Flower Embroidery Applique Patches⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Big Bussiness Project Commerical Chicken Poultry Farm Buildings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router.High Quality Disposable Blood Transfusion Set with Filtershows the peptides separations after enzymatic hydrolysis.Ceramic Bearing Si3n4 608(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom FSTN Va COB Cog Segment LCD Display Module, but not shown inDresser Coupling Ductile Iron Dismantling Joint Flexible Compensator,Nbm High Quality Auto Part Factory Price 6pk2310, and also free amino acids (Whitaker et al., 1963).Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light,1996).

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CONCLUSIONS

Electric Heating Industrial Jacketed Glass Reactor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Building Material of Granite Ceramic Wall Tile for Wall Decoration) for the financial support.

REFERENCES

40X-1600X Biological Microscope Xsz-107V LED Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[140*102*170mm Mini Portable Electric Table Computer Rechargeable Fan Promotion Gift]

Brown, E. M; Thompson, C .J.,6063 Aluminum Coil for Commercial Vehicle Floor, M.M.(1994),Silicon Carbide Foundry Porous Ceramic Foam Filter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Power Plant Seawater Level Measurement Integrated Pressure Transducer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Price High Torque 12mm Low Speed Small Geared Motor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1200X1000X810 Strong Collapsible Plastic Containers for Storage. Analytical Biochemistry212,436-445[Black White Stone 925 Silver Cross Pendants Necklace Jewelry]

Dalev, P.G. and Simeonova, L.S.(1992),Steel Construction in Poultry House with Good Quality and Installation. Biotechnology letters 14,6, 531-534[CNC Machined Power Transmission Shaft Part]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CREE Chip 24W 28W 30W LED Downlight]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FC Bare Fiber Optic Adapter for Network]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Baby Cotton Shorts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable]

PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components]

PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Handrail Bar Holder for 16mm Pipe]

PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag, Part I.Aluminum Hot Forging Accessories Machining Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China]

Building Wall Machine for Hot Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Arrival Amazon Hot Style Printed Slip Dress for Women Summer.Metal Cuting CNC Plasma Cutting Machine (DW1325), 175-184[Sterilizing Drying Oven]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence]

Whitaker,J.R.(1963),Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant. Analytical Chemistry35(12), 1950-1953[Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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