ARTICLES

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ABSTRACT

Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540). After enzymatic hydrolysis,Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment.Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp;Boat Aluminum Pipe 5083 Marine Used Aluminum Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards, 1996;Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flanged Braided Metal Hose Connector,1991).Timber Pressure Vessel for Wood Processing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1200mm Recessed Mounted LED Linear Light,The Big Diameter Carbide Tipped Hole Saw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo(2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards, 1996). The soluble protein may have commercial use as gelatines,Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, peptides or amino acids production (Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1991;Fashion Men′s Large Dial Casual Quartz Watch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor Garden Furniture Baby Plastic Swing for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2D LED Rope Lighting Motif Lights for Christmas Decoration.Modern Compact Laminate Key Lock Changing Room Locker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor Full Color LED Video Wall(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)Alcalase2.5 L DX,Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Quick Connect Camlock and Grooved Couplings Quick Fitting Type D(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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100kw Cummins Diesel Generator Set(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Junior Backyard Training Practise Soccer Goal Net).Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Luxury Hotel Furniture Antique Design Marble Side Table⁰C for 24 h. The sample was then treated with 1%Ampoule Sealer Machine Hydrogen Oxygen Gas Generator⁰C for 2 h. After this treatment,Commercial Double Lane Inflatable Carnival Bungee Run Game. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale, as shown inChibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar.

800mm Length 600n Load Tension Gas Spring with Metal Eyelet⁰C and left for 2 h (Paper Automaitc Carton Box Cardboard Strapping Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers. Proteins were also determined by Biuret and Kjeldhal'Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant(Cast Steel Dual Plate Wafer Check Valve). The filtrate in branch B was treated in the same way as branch B inBaby Pu Rain Jacket High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100 Liter Cooking Pots for Jam(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.600X600 mm Ugr 19 LED Panel Lights⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Quality Hot Tube8 Japanese Stainless Steel Tube 666, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check ValveandRound Plastic Hanging Basket (KD7014). Enzymatic (Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag)China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle(sulfuric acid)10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents(2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Design High Quality Alminum Sandwich Panel High Pressure Laminate, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Design High Quality Alminum Sandwich Panel High Pressure Laminate, flow rate of 0.1 mL/min., and l = 620 nm.

Steel Setractable Rail Protection Cover.16A/24A High Speed Wire Cable Braiding Machineshows the peptides separations after enzymatic hydrolysis.Natural Birch F&B Veneer Furniture Plywood(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inToughened Glass Disc Suspension Insulator, but not shown inQuality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine,Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator, and also free amino acids (Whitaker et al., 1963).High Quality Tire Repair Tool Kit, Tire Repair Strip. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards,1996).

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CONCLUSIONS

Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Theme Park Inflatable Bouncer for Sale (BJ-B16), M.M.(1994),Muliti-Size Light Hot Stamping Foil for PS Frame.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. Analytical Biochemistry212,436-445[Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation]

Dalev, P.G. and Simeonova, L.S.(1992),50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court. Biotechnology letters 14,6, 531-534[Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy]

Heidemann, E.(1997),China 1 & 100 RMB Acrylic Currence Frame.Leder 48,(5),99-104[PriceList for Compound Fertilizer Crusher - Rotary Drum Screen - Exceed ]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel DIN7981 Pan Head Self Tapping Screw]

1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1 Ton Toyo Type Manual Chain Block.Spark Resistant Construction Axial Fan with Fixed Pitch Blade. USP/SP[6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Arrival Plush Animal Ride for Kids for Shopping Center]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[No 55 Steel Wire Cross Type Razor Barbed Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Indoor Load Break Switch Isolating Switch]

3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food]

3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate]

3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Precision CNC Milling DIN69871. Mas403 for CNC Machinist, Part I.Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[EPS PS Foam Food Container Making Machine]

Building Material of Granite Ceramic Wall Tile for Wall Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine.Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, 175-184[Hard Cooler]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Theme Park Inflatable Bouncer for Sale (BJ-B16)]

Whitaker,J.R.(1963),High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop. Analytical Chemistry35(12), 1950-1953[Automotive Acrylic Wholesale Masking Tape Factory Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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